Transgenic pea with improved tolerance to Pea Enation Mosaic Virus.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F09%3A%230000151" target="_blank" >RIV/26784246:_____/09:#0000151 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Transgenic pea with improved tolerance to Pea Enation Mosaic Virus.
Popis výsledku v původním jazyce
Pea Enation Mosaic Virus (PEMV) and Pea Seed-borne Mosaic Virus (PSbMV) represent very important economical risks for pea growers by 10-25% decrease of seed yield. In our study, the induction of virus resistance / tolerance in transgenic pea lines were developed by the introgression of full length as well as fragments of cDNA of the PEMV and PSbMV viral coat proteins which were cloned in sense and antisense orientations into pGreenII plasmid (John Innes Centre, Norwich, UK). The level of virus replication after mechanical inoculation was monitored by time-sequenced DAS-ELISA. In transgenic pea lines T3 with PSbMV coat protein fragments the concentration of virus RNA was after 14 days transiently enhanced (in average up to 125%), but after 28 days the mean of RNA concentration was 37% as compared to the control non-transgenic inoculated plants (100%).
Název v anglickém jazyce
Transgenic pea with improved tolerance to Pea Enation Mosaic Virus.
Popis výsledku anglicky
Pea Enation Mosaic Virus (PEMV) and Pea Seed-borne Mosaic Virus (PSbMV) represent very important economical risks for pea growers by 10-25% decrease of seed yield. In our study, the induction of virus resistance / tolerance in transgenic pea lines were developed by the introgression of full length as well as fragments of cDNA of the PEMV and PSbMV viral coat proteins which were cloned in sense and antisense orientations into pGreenII plasmid (John Innes Centre, Norwich, UK). The level of virus replication after mechanical inoculation was monitored by time-sequenced DAS-ELISA. In transgenic pea lines T3 with PSbMV coat protein fragments the concentration of virus RNA was after 14 days transiently enhanced (in average up to 125%), but after 28 days the mean of RNA concentration was 37% as compared to the control non-transgenic inoculated plants (100%).
Klasifikace
Druh
C - Kapitola v odborné knize
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2009
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název knihy nebo sborníku
White book genetically modified crops
ISBN
978-80-86668-05-3
Počet stran výsledku
2
Strana od-do
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Počet stran knihy
96
Název nakladatele
Biology Centre of the Academy of Sciences of the Czech Republic
Místo vydání
České Budějovice
Kód UT WoS kapitoly
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