Marker assisted pea breeding: eIF4E allele speci?c markers to pea seed-borne mosaic virus (PSbMV) resistance.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F10%3A%230000257" target="_blank" >RIV/26784246:_____/10:#0000257 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Marker assisted pea breeding: eIF4E allele speci?c markers to pea seed-borne mosaic virus (PSbMV) resistance.

Popis výsledku v původním jazyce

We have analyzed for variation in the eIF4E genomic sequences from 43 pea varieties and breeding lines, reported as donors of resistance. This enabled a comprehensive investigation of the eIF4E gene structure and mutations responsible for PSbMV resistance were identi?ed. Subsequently, PCR-based and gene-speci?c single nucleotide polymorphism and co-dominant amplicon length polymorphism markers were developed. All together 60 accessions were analyzed using sequence data and/or allele speci?c DNA markers.Developed allele speci?c markers were reproducibly ampli?ed across a broad spectra of pea varieties and breeding lines. These were found to be 100% accurate in detecting the presence of the respective alleles when compared to symptomology and ELISA, testing (74% reliable). Hence, these molecular markers will substantially speed-up PSbMV diagnosis and resistance breeding processes in pea.

Název v anglickém jazyce

Marker assisted pea breeding: eIF4E allele speci?c markers to pea seed-borne mosaic virus (PSbMV) resistance.

Popis výsledku anglicky

We have analyzed for variation in the eIF4E genomic sequences from 43 pea varieties and breeding lines, reported as donors of resistance. This enabled a comprehensive investigation of the eIF4E gene structure and mutations responsible for PSbMV resistance were identi?ed. Subsequently, PCR-based and gene-speci?c single nucleotide polymorphism and co-dominant amplicon length polymorphism markers were developed. All together 60 accessions were analyzed using sequence data and/or allele speci?c DNA markers.Developed allele speci?c markers were reproducibly ampli?ed across a broad spectra of pea varieties and breeding lines. These were found to be 100% accurate in detecting the presence of the respective alleles when compared to symptomology and ELISA, testing (74% reliable). Hence, these molecular markers will substantially speed-up PSbMV diagnosis and resistance breeding processes in pea.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Breeding

ISSN

1380-3743

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

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Kód UT WoS článku

000282426600005

EID výsledku v databázi Scopus

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