Determination of T-2 and HT-2 Toxins in Seed of Milk Thistle [<i>Silybum marianum</i> (L.) Gaertn.] Using Immunoaffinity Column by UPLC-MS/MS
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F22%3AN0000064" target="_blank" >RIV/26784246:_____/22:N0000064 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.mdpi.com/2072-6651/14/4/258" target="_blank" >https://www.mdpi.com/2072-6651/14/4/258</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/toxins14040258" target="_blank" >10.3390/toxins14040258</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of T-2 and HT-2 Toxins in Seed of Milk Thistle [<i>Silybum marianum</i> (L.) Gaertn.] Using Immunoaffinity Column by UPLC-MS/MS
Popis výsledku v původním jazyce
Milk thistle [Silybum marianum (L.) Gaertn.] achieved a significant increase in interest over the past few years from local and foreign pharmaceutical corporations. The silymarin complex of constituents extracted from milk thistle achenes provides compelling health benefits primarily thanks to antioxidant activities and hepatoprotective effects. However, consuming mycotoxin-contaminated plant material can cause immunosuppression and hepatotoxic problems. The aim of this study was to develop and validate a method for the determination of mycotoxin content in milk thistle. Fusarium toxins as T-2 and HT-2 toxins in grown milk thistle harvested from a breeding station in the Czech Republic during 2020–2021 were studied. The analysis of T-2 and HT-2 toxins was performed by UPLC-MS/MS after immunoaffinity columns EASI-EXTRACT® T-2 & HT-2 clean up. All analysed samples of milk thistle were contaminated with T-2 toxin and HT-2 toxin. The content of T-2 toxin in the samples from 2020 was in the range of 122.7–290.2 µg/kg and HT-2 toxin 157.0–319.0 µg/kg. In 2021, the content of T-2 toxin was in the range of 28.8–69.9 µg/kg and HT-2 toxin was 24.2–75.4 µg/kg. The results show that the climatic conditions of the year of harvesting have a highly statistically significant effect on the content of T-2 and HT-2 toxins in milk thistle.
Název v anglickém jazyce
Determination of T-2 and HT-2 Toxins in Seed of Milk Thistle [<i>Silybum marianum</i> (L.) Gaertn.] Using Immunoaffinity Column by UPLC-MS/MS
Popis výsledku anglicky
Milk thistle [Silybum marianum (L.) Gaertn.] achieved a significant increase in interest over the past few years from local and foreign pharmaceutical corporations. The silymarin complex of constituents extracted from milk thistle achenes provides compelling health benefits primarily thanks to antioxidant activities and hepatoprotective effects. However, consuming mycotoxin-contaminated plant material can cause immunosuppression and hepatotoxic problems. The aim of this study was to develop and validate a method for the determination of mycotoxin content in milk thistle. Fusarium toxins as T-2 and HT-2 toxins in grown milk thistle harvested from a breeding station in the Czech Republic during 2020–2021 were studied. The analysis of T-2 and HT-2 toxins was performed by UPLC-MS/MS after immunoaffinity columns EASI-EXTRACT® T-2 & HT-2 clean up. All analysed samples of milk thistle were contaminated with T-2 toxin and HT-2 toxin. The content of T-2 toxin in the samples from 2020 was in the range of 122.7–290.2 µg/kg and HT-2 toxin 157.0–319.0 µg/kg. In 2021, the content of T-2 toxin was in the range of 28.8–69.9 µg/kg and HT-2 toxin was 24.2–75.4 µg/kg. The results show that the climatic conditions of the year of harvesting have a highly statistically significant effect on the content of T-2 and HT-2 toxins in milk thistle.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10700 - Other natural sciences
Návaznosti výsledku
Projekt
<a href="/cs/project/QK1910302" target="_blank" >QK1910302: Zpracování vedlejších produktů z lisování semen olejnin na nové výrobky s nutričními a zdravotními přínosy</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
TOXINS
ISSN
2072-6651
e-ISSN
2072-6651
Svazek periodika
14
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
12
Strana od-do
258
Kód UT WoS článku
000786002900001
EID výsledku v databázi Scopus
2-s2.0-85128614722