New Low Morphine Opium Poppy Genotype Obtained by TILLING Approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F23%3AN0000002" target="_blank" >RIV/26784246:_____/23:N0000002 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12220/23:43906658 RIV/26791251:_____/23:N0000056

Výsledek na webu

<a href="https://www.mdpi.com/2223-7747/12/5/1077" target="_blank" >https://www.mdpi.com/2223-7747/12/5/1077</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/plants12051077" target="_blank" >10.3390/plants12051077</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New Low Morphine Opium Poppy Genotype Obtained by TILLING Approach

Popis výsledku v původním jazyce

The opium poppy’s ability to produce various alkaloids is both useful and problematic. Breeding of new varieties with varying alkaloid content is therefore an important task. In this paper, the breeding technology of new low morphine poppy genotypes, based on a combination of a TILLING approach and single-molecule real-time NGS sequencing, is presented. Verification of the mutants in the TILLING population was obtained using RT-PCR and HPLC methods. Only three of the single-copy genes of the morphine pathway among the eleven genes were used for the identification of mutant genotypes. Point mutations were obtained only in one gene (CNMT) while an insertion was obtained in the other (SalAT). Only a few expected transition SNPs from G:C to A:T were obtained. In the low morphine mutant genotype, the production of morphine was decreased to 0.1% from 1.4% in the original variety. A comprehensive description of the breeding process, a basic characterization of the main alkaloid content, and a gene expression profile for the main alkaloid-producing genes is provided. Difficulties with the TILLING approach are also described and discussed.

Název v anglickém jazyce

New Low Morphine Opium Poppy Genotype Obtained by TILLING Approach

Popis výsledku anglicky

The opium poppy’s ability to produce various alkaloids is both useful and problematic. Breeding of new varieties with varying alkaloid content is therefore an important task. In this paper, the breeding technology of new low morphine poppy genotypes, based on a combination of a TILLING approach and single-molecule real-time NGS sequencing, is presented. Verification of the mutants in the TILLING population was obtained using RT-PCR and HPLC methods. Only three of the single-copy genes of the morphine pathway among the eleven genes were used for the identification of mutant genotypes. Point mutations were obtained only in one gene (CNMT) while an insertion was obtained in the other (SalAT). Only a few expected transition SNPs from G:C to A:T were obtained. In the low morphine mutant genotype, the production of morphine was decreased to 0.1% from 1.4% in the original variety. A comprehensive description of the breeding process, a basic characterization of the main alkaloid content, and a gene expression profile for the main alkaloid-producing genes is provided. Difficulties with the TILLING approach are also described and discussed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Projekt

<a href="/cs/project/QK1810391" target="_blank" >QK1810391: Využití technik genomiky a transkriptomiky k tvorbě genových zdrojů a výchozích materiálů máku se specifickými vlastnostmi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plants-Basel

ISSN

2223-7747

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

1077-1088

Kód UT WoS článku

000947057400001

EID výsledku v databázi Scopus

2-s2.0-85150223994