Rep-PCR-based typing as a tool for tracking of MRSA infection origin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26788462%3A_____%2F12%3A%230000613" target="_blank" >RIV/26788462:_____/12:#0000613 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/49608851:_____/12:#0000559

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Rep-PCR-based typing as a tool for tracking of MRSA infection origin

Popis výsledku v původním jazyce

A performance of rep-PCR fi ngerprinting method with the (GTG)5 primer was explored in order to evaluate its practical application for confi rmation the source of human staphylococci infection. A laboratory worker daily working with the MRSA and Staphylococcus aureus strains has been tested positively for MRSA nosocomial infection. The collection of nine MRSA strains held in the laboratory has been typed with the rep-PCR method. Analysis of the fi ngerprints with the unweighted pairgroup method using arithmetic averages (UPGMA) clustering method revealed presence of six strain clusters with similarity lower than 85%. The fi ngerprint of MRSA strain infecting the human diff ered signifi cantly from remaining fi ngerprints. This provided clear evidence that MRSA strain infecting the personal did not come from the laboratory strains collection. Our experimental results showed rep-PCR with the (GTG)5 primer as an eff ective tool applicable for diff erentiation of individual S. aureus strai

Název v anglickém jazyce

Rep-PCR-based typing as a tool for tracking of MRSA infection origin

Popis výsledku anglicky

A performance of rep-PCR fi ngerprinting method with the (GTG)5 primer was explored in order to evaluate its practical application for confi rmation the source of human staphylococci infection. A laboratory worker daily working with the MRSA and Staphylococcus aureus strains has been tested positively for MRSA nosocomial infection. The collection of nine MRSA strains held in the laboratory has been typed with the rep-PCR method. Analysis of the fi ngerprints with the unweighted pairgroup method using arithmetic averages (UPGMA) clustering method revealed presence of six strain clusters with similarity lower than 85%. The fi ngerprint of MRSA strain infecting the human diff ered signifi cantly from remaining fi ngerprints. This provided clear evidence that MRSA strain infecting the personal did not come from the laboratory strains collection. Our experimental results showed rep-PCR with the (GTG)5 primer as an eff ective tool applicable for diff erentiation of individual S. aureus strai

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Universitatis Agriculturae et Silviculturae Mendeleianae Brunensis

ISSN

1211-8516

e-ISSN

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Svazek periodika

60

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

251-256

Kód UT WoS článku

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EID výsledku v databázi Scopus

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