Ribotyping of Clostridioides difficile in the Liberec Regional Hospital: a tertiary health care facility

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F27283933%3A_____%2F22%3AN0000005" target="_blank" >RIV/27283933:_____/22:N0000005 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/23:43924370 RIV/00216208:11150/23:10451187 RIV/00179906:_____/23:10451187 RIV/00064173:_____/23:43924370 RIV/75010330:_____/23:00014471

Výsledek na webu

<a href="https://link.springer.com/content/pdf/10.1007/s12223-022-01021-z.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007/s12223-022-01021-z.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-022-01021-z" target="_blank" >10.1007/s12223-022-01021-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ribotyping of Clostridioides difficile in the Liberec Regional Hospital: a tertiary health care facility

Popis výsledku v původním jazyce

The ribotyping of Clostridioides difficile is one of the basic methods of molecular epidemiology for monitoring the spread of C. difficile infections. In the Czech Republic, this procedure is mainly available in university hospitals. The introduction of ribotyping in a tertiary health care facility such as Liberec Regional Hospital not only increases safety in the facility but also supports regional professional development. In our study, 556 stool samples collected between June 2017 and June 2018 were used for C. difficile infection screening, followed by cultivation, toxinotyping, and ribotyping of positive samples. The toxinotyping of 96 samples revealed that 44.8% of typed strains could produce toxins A and B encoded by tcdA and tcdB, respectively. The ribotyping of the same samples revealed two epidemic peaks, caused by the regionally most prevalent ribotype 176 (n = 30, 31.3). C. difficile infection incidence ranged between 5.5 and 4.2 cases per 10,000 patient-bed days. Molecular diagnostics and molecular epidemiology are the two most developing parts of clinical laboratories. The correct applications of molecular methods help ensure greater safety in hospitals.

Název v anglickém jazyce

Ribotyping of Clostridioides difficile in the Liberec Regional Hospital: a tertiary health care facility

Popis výsledku anglicky

The ribotyping of Clostridioides difficile is one of the basic methods of molecular epidemiology for monitoring the spread of C. difficile infections. In the Czech Republic, this procedure is mainly available in university hospitals. The introduction of ribotyping in a tertiary health care facility such as Liberec Regional Hospital not only increases safety in the facility but also supports regional professional development. In our study, 556 stool samples collected between June 2017 and June 2018 were used for C. difficile infection screening, followed by cultivation, toxinotyping, and ribotyping of positive samples. The toxinotyping of 96 samples revealed that 44.8% of typed strains could produce toxins A and B encoded by tcdA and tcdB, respectively. The ribotyping of the same samples revealed two epidemic peaks, caused by the regionally most prevalent ribotype 176 (n = 30, 31.3). C. difficile infection incidence ranged between 5.5 and 4.2 cases per 10,000 patient-bed days. Molecular diagnostics and molecular epidemiology are the two most developing parts of clinical laboratories. The correct applications of molecular methods help ensure greater safety in hospitals.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30101 - Human genetics

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

315-320

Kód UT WoS článku

000912647600001

EID výsledku v databázi Scopus

2-s2.0-85143218252