Phenol-driven cometabolic degradation of cis-1,2-dichloroethene (cDCE): insights from Acinetobacter pittii and Ectopseudomonas alcaliphila

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F27283933%3A_____%2F25%3AN0000026" target="_blank" >RIV/27283933:_____/25:N0000026 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1186/s12302-025-01237-z" target="_blank" >https://link.springer.com/article/10.1186/s12302-025-01237-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12302-025-01237-z" target="_blank" >10.1186/s12302-025-01237-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phenol-driven cometabolic degradation of cis-1,2-dichloroethene (cDCE): insights from Acinetobacter pittii and Ectopseudomonas alcaliphila

Popis výsledku v původním jazyce

Accumulation of xenobiotic chlorinated ethenes (CEs) at legacy industrial soil and groundwater sites around the world is a pressing environmental and public health issue. Understanding the biochemical pathways through which microorganisms degrade cDCE is key to developing cost-effective, sustainable bioremediation strategies for CE contamination. Two strains, Acinetobacter pittii CEP14 and Ectopseudomonas alcaliphila JAB1, isolated from contaminated industrial sites, have demonstrated the ability to cometabolically degrade cDCE in the presence of phenol. In this study, we integrate transcriptomics, using differential gene expression analysis to pinpoint genes induced during cDCE co‐metabolism, with proteomics to confirm protein‐level expression. We use heterologous expression experiments to demonstrate that phenol monooxygenase is responsible for oxidising cDCE in both strains. Furthermore, we show that CEP14 and JAB1 α-subunits share 71.4% identity with each other but only 14.6–26.5% identity with established monooxygenases with known cDCE-oxidising activity, highlighting the diversity of enzymes that may be capable of cometabolic cDCE degradation. Finally, we hypothesise on a two-branch phenol monooxygenase-mediated cDCE degradation pathway in which the chemical degradative intermediates 2,2-dichloroacetaldehyde and cDCE epoxides are formed. This study sheds light on the biochemical mechanisms by which monoaromatic compounds can enhance the biodegradation of cDCE and demonstrates the potential utilisation of strains CEP14 and JAB1 for the biodegradation of cDCE.

Název v anglickém jazyce

Phenol-driven cometabolic degradation of cis-1,2-dichloroethene (cDCE): insights from Acinetobacter pittii and Ectopseudomonas alcaliphila

Popis výsledku anglicky

Accumulation of xenobiotic chlorinated ethenes (CEs) at legacy industrial soil and groundwater sites around the world is a pressing environmental and public health issue. Understanding the biochemical pathways through which microorganisms degrade cDCE is key to developing cost-effective, sustainable bioremediation strategies for CE contamination. Two strains, Acinetobacter pittii CEP14 and Ectopseudomonas alcaliphila JAB1, isolated from contaminated industrial sites, have demonstrated the ability to cometabolically degrade cDCE in the presence of phenol. In this study, we integrate transcriptomics, using differential gene expression analysis to pinpoint genes induced during cDCE co‐metabolism, with proteomics to confirm protein‐level expression. We use heterologous expression experiments to demonstrate that phenol monooxygenase is responsible for oxidising cDCE in both strains. Furthermore, we show that CEP14 and JAB1 α-subunits share 71.4% identity with each other but only 14.6–26.5% identity with established monooxygenases with known cDCE-oxidising activity, highlighting the diversity of enzymes that may be capable of cometabolic cDCE degradation. Finally, we hypothesise on a two-branch phenol monooxygenase-mediated cDCE degradation pathway in which the chemical degradative intermediates 2,2-dichloroacetaldehyde and cDCE epoxides are formed. This study sheds light on the biochemical mechanisms by which monoaromatic compounds can enhance the biodegradation of cDCE and demonstrates the potential utilisation of strains CEP14 and JAB1 for the biodegradation of cDCE.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

—

OECD FORD obor

30304 - Public and environmental health

Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Sciences Europe

ISSN

—

e-ISSN

2190-4715

Svazek periodika

37

Číslo periodika v rámci svazku

196

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

15

Strana od-do

—

Kód UT WoS článku

001609529600004

EID výsledku v databázi Scopus

2-s2.0-105021089092