Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F27661989%3A_____%2F26%3A10003560" target="_blank" >RIV/27661989:_____/26:10003560 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00064211:_____/26:W0000092 RIV/00064190:_____/26:10001450

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1201971226000792" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1201971226000792</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijid.2026.108444" target="_blank" >10.1016/j.ijid.2026.108444</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic

Popis výsledku v původním jazyce

Background and aims: Clostridioides difficile ribotypes (RTs) 001 and 176 dominate the epidemiology of C. difficile infection (CDI) in the Czech Republic. We applied whole-genome sequencing (WGS) and broad-range antimicrobial susceptibility testing (AST) to provide detailed characterization of these lineages. Methods: Between October and December 2019, 22 hospitals participated. CDI isolates were characterized by PCR ribotyping, toxin gene detection and AST to metronidazole, vancomycin and moxifloxacin. WGS and broad-range AST were performed on selected RT001 and RT176 isolates. Results: The mean CDI incidence was 5.1 cases/10,000 patient-days. Isolates and epidemiological data were available for 495/524 (94.5%) CDIs. RT001 ( n = 166, 33.5%), RT014 ( n = 59, 11.9%) RT176 ( n = 51, 10.3%) were the most prevalent. Among 21 RT001 isolates, wgMLST (3745 loci) revealed 0-120 allele differences and 21 genomic inserts, eight of which carried antimicrobial resistance (AMR) genes, including cfrB, which encodes linezolid resistance. In 13 RT176 isolates, wgMLST (3298 loci) showed 0-9 allele differences and 11 inserts, eight with AMR genes. The wgMLST confirmed clonal relatedness of RT001 and RT176 isolates from different hospitals (0-3 allelic differences), yet with variation in acquired AMR gene content. Differences between genotype and expected phenotype were observed in PnimBG and metronidazole, cfrE and linezolid and tetgenes and tetracycline. Conclusion: RT001 and RT176 predominate in the Czech Republic, and WGS confirmed their inter-hospital clonal relatedness. Importantly, the emergence of linezolid-resistant RT001 strains was detected in nine hospitals. The inclusion of AMR genes in genetic relatedness analysis showed higher discriminatory power compared to cgMLST or wgMLST alone. (c) 2026 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

Název v anglickém jazyce

Clonal relatedness and emerging linezolid resistance in Clostridioides difficile ribotypes 001 and 176 in the Czech Republic

Popis výsledku anglicky

Background and aims: Clostridioides difficile ribotypes (RTs) 001 and 176 dominate the epidemiology of C. difficile infection (CDI) in the Czech Republic. We applied whole-genome sequencing (WGS) and broad-range antimicrobial susceptibility testing (AST) to provide detailed characterization of these lineages. Methods: Between October and December 2019, 22 hospitals participated. CDI isolates were characterized by PCR ribotyping, toxin gene detection and AST to metronidazole, vancomycin and moxifloxacin. WGS and broad-range AST were performed on selected RT001 and RT176 isolates. Results: The mean CDI incidence was 5.1 cases/10,000 patient-days. Isolates and epidemiological data were available for 495/524 (94.5%) CDIs. RT001 ( n = 166, 33.5%), RT014 ( n = 59, 11.9%) RT176 ( n = 51, 10.3%) were the most prevalent. Among 21 RT001 isolates, wgMLST (3745 loci) revealed 0-120 allele differences and 21 genomic inserts, eight of which carried antimicrobial resistance (AMR) genes, including cfrB, which encodes linezolid resistance. In 13 RT176 isolates, wgMLST (3298 loci) showed 0-9 allele differences and 11 inserts, eight with AMR genes. The wgMLST confirmed clonal relatedness of RT001 and RT176 isolates from different hospitals (0-3 allelic differences), yet with variation in acquired AMR gene content. Differences between genotype and expected phenotype were observed in PnimBG and metronidazole, cfrE and linezolid and tetgenes and tetracycline. Conclusion: RT001 and RT176 predominate in the Czech Republic, and WGS confirmed their inter-hospital clonal relatedness. Importantly, the emergence of linezolid-resistant RT001 strains was detected in nine hospitals. The inclusion of AMR genes in genetic relatedness analysis showed higher discriminatory power compared to cgMLST or wgMLST alone. (c) 2026 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30230 - Other clinical medicine subjects

Projekt

—

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2026

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Infectious Diseases

ISSN

1201-9712

e-ISSN

1878-3511

Svazek periodika

165

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

nestránkováno

Kód UT WoS článku

001705165000001

EID výsledku v databázi Scopus

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