Reversible immobilisation of engineered molecules by Ni-NTA chelators.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13430%2F03%3A00002211" target="_blank" >RIV/44555601:13430/03:00002211 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Reversible immobilisation of engineered molecules by Ni-NTA chelators.

Popis výsledku v původním jazyce

Biomolecules modified by genetic engineering represent a new and powerful approach for obtaining simpler artificial structures with new or improved properties useful for biosensing purposes. In this paper graphite or gold working electrodes were obtainedby several deposition techniques as well as sputtering, screen-printing and chemical gold plating on copper paths. NH2 groups exposed out the surface were obtained by self assembled monolayers (SAM) of 3-aminopropyltriethoxysilane (3-APTES) on graphite screen printed electrodes or cysteamine on sputtered gold or chemically plated gold on copper. The electrochemical synthesis of the SAM mixed with conducting molecular wires of polianyline (PAN) was studied, obtaining higher deposition yields (increased 50 fold), lower treatment time (<1min with respect to 12h of the chemical treatment), and the ability to address the synthesis on only one electrode in a sensor ?-array. The synthesis of the Ni-NTA chelator followed the common scheme: a)gl

Název v anglickém jazyce

Reversible immobilisation of engineered molecules by Ni-NTA chelators.

Popis výsledku anglicky

Biomolecules modified by genetic engineering represent a new and powerful approach for obtaining simpler artificial structures with new or improved properties useful for biosensing purposes. In this paper graphite or gold working electrodes were obtainedby several deposition techniques as well as sputtering, screen-printing and chemical gold plating on copper paths. NH2 groups exposed out the surface were obtained by self assembled monolayers (SAM) of 3-aminopropyltriethoxysilane (3-APTES) on graphite screen printed electrodes or cysteamine on sputtered gold or chemically plated gold on copper. The electrochemical synthesis of the SAM mixed with conducting molecular wires of polianyline (PAN) was studied, obtaining higher deposition yields (increased 50 fold), lower treatment time (<1min with respect to 12h of the chemical treatment), and the ability to address the synthesis on only one electrode in a sensor ?-array. The synthesis of the Ni-NTA chelator followed the common scheme: a)gl

Druh

D - Stať ve sborníku

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F03%2F0659" target="_blank" >GA522/03/0659: Zdokonalení fotosyntetického biosenzoru a biologických testů pro rutinní detekci reziduí herbicidů a určení rizik pro necílové organismy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník z mezinárodní konference Bioelectrochemistry and Bioenergetics

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

222-223

Název nakladatele

Tip.G. Capponi srl.

Místo vydání

Firenze

Místo konání akce

Firenze, Italy

Datum konání akce

19. 6. 2003

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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