Elektrochemická mikrocela pro cílenou imobilizaci biomolekul

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F05%3A00003083" target="_blank" >RIV/44555601:13440/05:00003083 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Continuous flow micro-cell for electrochemical addressing of engineered bio-molecules

Popis výsledku v původním jazyce

A micro-cell consisting of a planar array of Au working electrodes, covered by a glass reservoir (600 nl capacity), connected with perpendicular capillaries and provided with an Ag/AgCl wire reference electrode (RE) has been manufactured. Addressed on-chip immobilization and sensing of biomolecules was demonstrated by the immobilization of histidine (HIS) tagged alkaline phosphatase (AP) under continuous flow (10_l/min) conditions on the base of electrochemically deposited multilayer (EDM) of cysteaminemodified by nitrilotriacetic acid (NTA). The presented method allows the immobilization of different biomolecules on the ready to-use reservoir covered chip without needs of manual biomolecule deposition. Further on, the detection of analytes can be performed using the electrochemical measurements. Since the methods of biomolecule deposition and sensing are both electrochemical, this allows simplifying the production technology using cheaper detection instruments compared to prevailing

Název v anglickém jazyce

Continuous flow micro-cell for electrochemical addressing of engineered bio-molecules

Popis výsledku anglicky

A micro-cell consisting of a planar array of Au working electrodes, covered by a glass reservoir (600 nl capacity), connected with perpendicular capillaries and provided with an Ag/AgCl wire reference electrode (RE) has been manufactured. Addressed on-chip immobilization and sensing of biomolecules was demonstrated by the immobilization of histidine (HIS) tagged alkaline phosphatase (AP) under continuous flow (10_l/min) conditions on the base of electrochemically deposited multilayer (EDM) of cysteaminemodified by nitrilotriacetic acid (NTA). The presented method allows the immobilization of different biomolecules on the ready to-use reservoir covered chip without needs of manual biomolecule deposition. Further on, the detection of analytes can be performed using the electrochemical measurements. Since the methods of biomolecule deposition and sensing are both electrochemical, this allows simplifying the production technology using cheaper detection instruments compared to prevailing

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F03%2F0659" target="_blank" >GA522/03/0659: Zdokonalení fotosyntetického biosenzoru a biologických testů pro rutinní detekci reziduí herbicidů a určení rizik pro necílové organismy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators. B - Chemical

ISSN

0925-4005

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

111-112

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

6

Strana od-do

317-322

Kód UT WoS článku

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EID výsledku v databázi Scopus

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