Zhodnocení různých ELISA metod pro sérodiagnostiku trichinelózy u lidí
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F06%3A00003405" target="_blank" >RIV/44555601:13440/06:00003405 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62157124:16170/06:00000519
Výsledek na webu
—
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
Popis výsledku v původním jazyce
Introduction. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis are
Název v anglickém jazyce
The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
Popis výsledku anglicky
Introduction. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis are
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EG - Zoologie
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/GD524%2F03%2FH133" target="_blank" >GD524/03/H133: Biologie, fylogeneze a ekologie parazitů na organismální a molekulární úrovni</a><br>
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2006
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Wiadomoąci Parazytologiczne
ISSN
0043-5163
e-ISSN
—
Svazek periodika
52
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
PL - Polská republika
Počet stran výsledku
8
Strana od-do
231-238
Kód UT WoS článku
—
EID výsledku v databázi Scopus
—