Cationic Carbosilane Dendrimers as Non-virial Vectors of Nucleic Acids (Oligonucleotide or sirNa) for Gene Therapy Purposes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F13%3A43885365" target="_blank" >RIV/44555601:13440/13:43885365 - isvavai.cz</a>

Výsledek na webu

<a href="http://rsclive3.rsc.org/publishing/ebooks/2013/9781849736114.asp" target="_blank" >http://rsclive3.rsc.org/publishing/ebooks/2013/9781849736114.asp</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/9781849737296-00040" target="_blank" >10.1039/9781849737296-00040</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cationic Carbosilane Dendrimers as Non-virial Vectors of Nucleic Acids (Oligonucleotide or sirNa) for Gene Therapy Purposes

Popis výsledku v původním jazyce

A new approach to develop novel non-viral vector for HIV inhibition based on carbosilane dendrimers is described. Cationic ammonium-terminated carbosilane dendrimers have been synthesized via alcoholysis of dendritic Si-Cl bonds or hydrosylilation reactions with dendritic Si-H bonds. Ammonium-terminated carbosilane dendrimers are able to form dendriplexes which showed good biocompatibility profiles in vitro, and protect nucleic acids from binding to serum proteins or RNases and degradation. Finally, they were found to be a successful and efficient vehicle for the transport and delivery of genetic material (ODN or siRNA) to a wide range of immune cells, and according to this, a specific reduction of HIV p24 antigen release by anti-HIV ODN or siRNA was observed. Therefore, the carbosilane dendrimers presented here are in general good candidates as non-viral vectors in the context of biological applications of small nucleic acids, and in particular, they may present a new interesting appr

Název v anglickém jazyce

Cationic Carbosilane Dendrimers as Non-virial Vectors of Nucleic Acids (Oligonucleotide or sirNa) for Gene Therapy Purposes

Popis výsledku anglicky

A new approach to develop novel non-viral vector for HIV inhibition based on carbosilane dendrimers is described. Cationic ammonium-terminated carbosilane dendrimers have been synthesized via alcoholysis of dendritic Si-Cl bonds or hydrosylilation reactions with dendritic Si-H bonds. Ammonium-terminated carbosilane dendrimers are able to form dendriplexes which showed good biocompatibility profiles in vitro, and protect nucleic acids from binding to serum proteins or RNases and degradation. Finally, they were found to be a successful and efficient vehicle for the transport and delivery of genetic material (ODN or siRNA) to a wide range of immune cells, and according to this, a specific reduction of HIV p24 antigen release by anti-HIV ODN or siRNA was observed. Therefore, the carbosilane dendrimers presented here are in general good candidates as non-viral vectors in the context of biological applications of small nucleic acids, and in particular, they may present a new interesting appr

Druh

C - Kapitola v odborné knize

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Dendrimers in Biomedical Applications

ISBN

978-1-84973-611-4

Počet stran výsledku

15

Strana od-do

40-55

Počet stran knihy

200

Název nakladatele

Royal Society of Chemistry

Místo vydání

London

Kód UT WoS kapitoly

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