Phosphonium carbosilane dendrimers for biomedical applications - synthesis, characterization and cytotoxicity evaluation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F17%3A43892841" target="_blank" >RIV/44555601:13440/17:43892841 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985858:_____/17:00473749

Výsledek na webu

<a href="http://pubs.rsc.org/en/content/articlepdf/2017/ra/c7ra01845b" target="_blank" >http://pubs.rsc.org/en/content/articlepdf/2017/ra/c7ra01845b</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c7ra01845b" target="_blank" >10.1039/c7ra01845b</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phosphonium carbosilane dendrimers for biomedical applications - synthesis, characterization and cytotoxicity evaluation

Popis výsledku v původním jazyce

We report the synthesis and cytotoxicity evaluation of a completely new class of cationic carbosilane dendrimers functionalized with several different phosphonium peripheral groups and an ammonium functionalised one as a reference. The carbosilane dendrimers with NMe3, PMe3, P(Et-2)(2)(CH2)(3)OH, PBu3, P(C6H4-OMe)(3) and P(Ph)(3) peripheral substituents were synthesized, thoroughly characterized and modelled by computer simulations. The cytotoxicities of the dendrimers were investigated in vitro on three model cell lines (B14, BRL and NRK cells) by MTT and CV assay methods. Generally, the cytotoxicities of PMe3 carbosilane dendrimers were similar or slightly lower when compared with NMe3 dendrimers. The substitution of methyl groups in PMe3 carbosilane dendrimers with more hydrophobic and bulky alkyl substituents (PBu3 and P(Et-2)(2)(CH2)(3)OH dendrimers) resulted in an increase of cytotoxicity. The P(C6H4-OMe)(3) dendrimer showed exceptionally low cytotoxicity across all cell lines or assay methods used. Generally, phosphonium carbosilane dendrimers could represent a valuable alternative to ammonium ones in gene therapy applications due to comparable or lower cytotoxicities, the presence of positive charge for nucleic acid electrostatic binding and in the cases of P(C6H4-OMe)(3) and P(Ph)(3) dendrimers high potential of mitochondrial targeting.

Název v anglickém jazyce

Phosphonium carbosilane dendrimers for biomedical applications - synthesis, characterization and cytotoxicity evaluation

Popis výsledku anglicky

We report the synthesis and cytotoxicity evaluation of a completely new class of cationic carbosilane dendrimers functionalized with several different phosphonium peripheral groups and an ammonium functionalised one as a reference. The carbosilane dendrimers with NMe3, PMe3, P(Et-2)(2)(CH2)(3)OH, PBu3, P(C6H4-OMe)(3) and P(Ph)(3) peripheral substituents were synthesized, thoroughly characterized and modelled by computer simulations. The cytotoxicities of the dendrimers were investigated in vitro on three model cell lines (B14, BRL and NRK cells) by MTT and CV assay methods. Generally, the cytotoxicities of PMe3 carbosilane dendrimers were similar or slightly lower when compared with NMe3 dendrimers. The substitution of methyl groups in PMe3 carbosilane dendrimers with more hydrophobic and bulky alkyl substituents (PBu3 and P(Et-2)(2)(CH2)(3)OH dendrimers) resulted in an increase of cytotoxicity. The P(C6H4-OMe)(3) dendrimer showed exceptionally low cytotoxicity across all cell lines or assay methods used. Generally, phosphonium carbosilane dendrimers could represent a valuable alternative to ammonium ones in gene therapy applications due to comparable or lower cytotoxicities, the presence of positive charge for nucleic acid electrostatic binding and in the cases of P(C6H4-OMe)(3) and P(Ph)(3) dendrimers high potential of mitochondrial targeting.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20501 - Materials engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RSC Advances

ISSN

2046-2069

e-ISSN

—

Svazek periodika

2017

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

21

Strana od-do

18724-18744

Kód UT WoS článku

000399005500067

EID výsledku v databázi Scopus

2-s2.0-85016408382