6S-Like scr3559 RNA Affects Development and Antibiotic Production in Streptomyces coelicolor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F21%3A43896487" target="_blank" >RIV/44555601:13440/21:43896487 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/21:10434358

Výsledek na webu

<a href="https://www.mdpi.com/2076-2607/9/10/2004" target="_blank" >https://www.mdpi.com/2076-2607/9/10/2004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms9102004" target="_blank" >10.3390/microorganisms9102004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

6S-Like scr3559 RNA Affects Development and Antibiotic Production in Streptomyces coelicolor

Popis výsledku v původním jazyce

Regulatory RNAs control a number of physiological processes in bacterial cells. Here we report on a 6S-like RNA transcript (scr3559) that affects both development and antibiotic production in Streptomyces coelicolor. Its expression is enhanced during the transition to stationary phase. Strains that over-expressed the scr3559 gene region exhibited a shortened exponential growth phase in comparison with a control strain; accelerated aerial mycelium formation and spore maturation; alongside an elevated production of actinorhodin and undecylprodigiosin. These observations were supported by LC-MS analyses of other produced metabolites, including: germicidins, desferrioxamines, and coelimycin. A subsequent microarray differential analysis revealed increased expression of genes associated with the described morphological and physiological changes. Structural and functional similarities between the scr3559 transcript and 6S RNA, and its possible employment in regulating secondary metabolite production are discussed.

Název v anglickém jazyce

6S-Like scr3559 RNA Affects Development and Antibiotic Production in Streptomyces coelicolor

Popis výsledku anglicky

Regulatory RNAs control a number of physiological processes in bacterial cells. Here we report on a 6S-like RNA transcript (scr3559) that affects both development and antibiotic production in Streptomyces coelicolor. Its expression is enhanced during the transition to stationary phase. Strains that over-expressed the scr3559 gene region exhibited a shortened exponential growth phase in comparison with a control strain; accelerated aerial mycelium formation and spore maturation; alongside an elevated production of actinorhodin and undecylprodigiosin. These observations were supported by LC-MS analyses of other produced metabolites, including: germicidins, desferrioxamines, and coelimycin. A subsequent microarray differential analysis revealed increased expression of genes associated with the described morphological and physiological changes. Structural and functional similarities between the scr3559 transcript and 6S RNA, and its possible employment in regulating secondary metabolite production are discussed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganisms

ISSN

2076-2607

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

"nestrankovano"

Kód UT WoS článku

000713142400001

EID výsledku v databázi Scopus

2-s2.0-85115421282