Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: implications for deep geological repository

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46356088%3A_____%2F24%3AN0000016" target="_blank" >RIV/46356088:_____/24:N0000016 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24620/24:00012911

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11274-024-04069-w" target="_blank" >https://link.springer.com/article/10.1007/s11274-024-04069-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11274-024-04069-w" target="_blank" >10.1007/s11274-024-04069-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: implications for deep geological repository

Popis výsledku v původním jazyce

Bentonite is an integral part of the engineered barrier system (EBS) in deep geological repositories (DGR) for nuclear waste, but its indigenous microorganisms may jeopardize long-term EBS integrity. To predict microbial activity in DGRs, it is essential to understand microbial reactions to the early hot phase of DGR evolution. Two bentonites (BCV and MX-80) with varied bentonite/water ratios and saturation levels (compacted to 1600 kg.m− 3 dry density/powder/suspension), were subjected to heat (90–150 °C) and irradiation (0.4 Gy.h− 1) in the long-term experiments (up to 18 months). Molecular-genetic, microscopic, and cultivation-based techniques assessed microbial survivability. Exposure to 90 °C and 150 °C notably diminished microbial viability, irrespective of bentonite form, with negligible impacts from irradiation or sample type compared to temperature. Bentonite powder samples exhibited microbial recovery after 90 °C heating for up to 6 months but not 12 months in most cases; exposure to 150 °C had an even stronger effect. Further long-term experiments at additional temperatures combined with the mathematical prediction of temperature evolution in DGR are recommended to validate the possible evolution and spatial distribution of microbially depleted zones in bentonite buffer around the waste canisters and refine predictions of microbial effects over time in the DGR.

Název v anglickém jazyce

Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: implications for deep geological repository

Popis výsledku anglicky

Bentonite is an integral part of the engineered barrier system (EBS) in deep geological repositories (DGR) for nuclear waste, but its indigenous microorganisms may jeopardize long-term EBS integrity. To predict microbial activity in DGRs, it is essential to understand microbial reactions to the early hot phase of DGR evolution. Two bentonites (BCV and MX-80) with varied bentonite/water ratios and saturation levels (compacted to 1600 kg.m− 3 dry density/powder/suspension), were subjected to heat (90–150 °C) and irradiation (0.4 Gy.h− 1) in the long-term experiments (up to 18 months). Molecular-genetic, microscopic, and cultivation-based techniques assessed microbial survivability. Exposure to 90 °C and 150 °C notably diminished microbial viability, irrespective of bentonite form, with negligible impacts from irradiation or sample type compared to temperature. Bentonite powder samples exhibited microbial recovery after 90 °C heating for up to 6 months but not 12 months in most cases; exposure to 150 °C had an even stronger effect. Further long-term experiments at additional temperatures combined with the mathematical prediction of temperature evolution in DGR are recommended to validate the possible evolution and spatial distribution of microbially depleted zones in bentonite buffer around the waste canisters and refine predictions of microbial effects over time in the DGR.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

—

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

World journal of microbiology and biotechnology

ISSN

0959-3993

e-ISSN

1573-0972

Svazek periodika

40

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

19

Strana od-do

1-19

Kód UT WoS článku

001266641500001

EID výsledku v databázi Scopus

2-s2.0-85198120848