Male fertility restored by transplanting primordial germ cells into testes: A new way towards efficient transgenesis in chicken

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46356606%3A_____%2F17%3AN0000004" target="_blank" >RIV/46356606:_____/17:N0000004 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/17:00487318

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-017-14475-w" target="_blank" >https://www.nature.com/articles/s41598-017-14475-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-017-14475-w" target="_blank" >10.1038/s41598-017-14475-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Male fertility restored by transplanting primordial germ cells into testes: A new way towards efficient transgenesis in chicken

Popis výsledku v původním jazyce

The ongoing progress in primordial germ cell derivation and cultivation is opening new ways in reproductive biotechnology. This study tested whether functional sperm cells can be matured from genetically manipulated primordial germ cells after transplantation in adult testes and used to restore fertility. We show that spermatogenesis can be restored after mCherry-expressing or GFP-expressing primordial germ cells are transplantated into the testes of sterilized G(0) roosters and that mCherry-positive or GFP-positive non-chimeric transgenic G(1) offspring can be efficiently produced. Compared with the existing approaches to primordial germ cell replacement, this new technique eliminates the germ line chimerism of G(0) roosters and is, therefore, faster, more efficient and requires fewer animals. Furthermore, this is the only animal model, where the fate of primordial germ cells in infertile recipients can be studied.

Název v anglickém jazyce

Male fertility restored by transplanting primordial germ cells into testes: A new way towards efficient transgenesis in chicken

Popis výsledku anglicky

The ongoing progress in primordial germ cell derivation and cultivation is opening new ways in reproductive biotechnology. This study tested whether functional sperm cells can be matured from genetically manipulated primordial germ cells after transplantation in adult testes and used to restore fertility. We show that spermatogenesis can be restored after mCherry-expressing or GFP-expressing primordial germ cells are transplantated into the testes of sterilized G(0) roosters and that mCherry-positive or GFP-positive non-chimeric transgenic G(1) offspring can be efficiently produced. Compared with the existing approaches to primordial germ cell replacement, this new technique eliminates the germ line chimerism of G(0) roosters and is, therefore, faster, more efficient and requires fewer animals. Furthermore, this is the only animal model, where the fate of primordial germ cells in infertile recipients can be studied.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

<a href="/cs/project/GA15-23993S" target="_blank" >GA15-23993S: Cílená genová modifikace u drůbeže a resistence k novým typům retrovirů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Svazek periodika

7

Číslo periodika v rámci svazku

14246

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

—

Kód UT WoS článku

000413907000069

EID výsledku v databázi Scopus

2-s2.0-85032461218