DRAWING FIBROUS SCAFFOLDS WITH PRECISE STRUCTURE FOR TISSUE ENGINEERING
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24210%2F14%3A00003522" target="_blank" >RIV/46747885:24210/14:00003522 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/46747885:24410/14:00003522
Výsledek na webu
<a href="http://www.nanocon.eu/files/uploads/01/NANOCON" target="_blank" >http://www.nanocon.eu/files/uploads/01/NANOCON</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
DRAWING FIBROUS SCAFFOLDS WITH PRECISE STRUCTURE FOR TISSUE ENGINEERING
Popis výsledku v původním jazyce
This work is focused on evaluation of scaffolds made by drawing technique for the cell adhesion, viability and proliferation. With drawing we are able to produce very precise fibres with specified orientation from different polymers (PCL, PVA, PVB and others). This characteristic could be an advantage in tissues with ordered pattern. In the primary study the handmade fibres were tested for the cell growth and the results showed the potential of the cells to overgrow the gaps between the fibres. On account of these results further tests were necessary to be done with the precise machine-made fibrous scaffolds. The machine-made fibres are thinner, the gaps between the fibres are smaller and the whole structure is denser. In this study we focused on a planar scaffolds with fibres ordered either in one, in two or in three directions. Mice fibroblasts (3T3 Swiss Albino) were used for the in vitro experiment. The scaffolds were tested at day 1, 3, 7, 14, 21 and 28 after cell seeding. MTT assay, fluorescent microscopy and SEM analysis were used for the evaluation of cell adhesion, viability and proliferation. The results from microscopy analysis as well as from the MTT assay show, that the machine-made scaffolds are capable of supporting cellular attachment and proliferation during our in vitro tests. The scaffolds with crossed fibres show better results compared to fibres ordered in one direction. Nevertheless the one-direction fibres have a great potential in many tissue engineering applications, e.g. for muscles or neural tissue..
Název v anglickém jazyce
DRAWING FIBROUS SCAFFOLDS WITH PRECISE STRUCTURE FOR TISSUE ENGINEERING
Popis výsledku anglicky
This work is focused on evaluation of scaffolds made by drawing technique for the cell adhesion, viability and proliferation. With drawing we are able to produce very precise fibres with specified orientation from different polymers (PCL, PVA, PVB and others). This characteristic could be an advantage in tissues with ordered pattern. In the primary study the handmade fibres were tested for the cell growth and the results showed the potential of the cells to overgrow the gaps between the fibres. On account of these results further tests were necessary to be done with the precise machine-made fibrous scaffolds. The machine-made fibres are thinner, the gaps between the fibres are smaller and the whole structure is denser. In this study we focused on a planar scaffolds with fibres ordered either in one, in two or in three directions. Mice fibroblasts (3T3 Swiss Albino) were used for the in vitro experiment. The scaffolds were tested at day 1, 3, 7, 14, 21 and 28 after cell seeding. MTT assay, fluorescent microscopy and SEM analysis were used for the evaluation of cell adhesion, viability and proliferation. The results from microscopy analysis as well as from the MTT assay show, that the machine-made scaffolds are capable of supporting cellular attachment and proliferation during our in vitro tests. The scaffolds with crossed fibres show better results compared to fibres ordered in one direction. Nevertheless the one-direction fibres have a great potential in many tissue engineering applications, e.g. for muscles or neural tissue..
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
FP - Ostatní lékařské obory
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED3.1.00%2F14.0306" target="_blank" >ED3.1.00/14.0306: Inovativní výrobky a environmentální technologie</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
NANOCON 2014 - Conference Proceedings, 6th International Conference
ISBN
978-80-87294-53-6
ISSN
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e-ISSN
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Počet stran výsledku
6
Strana od-do
508-513
Název nakladatele
TANGER LTD, OSTRAVA
Místo vydání
Ostrava, CZECH REPUBLIC
Místo konání akce
Brno, CZECH REPUBLIC
Datum konání akce
1. 1. 2014
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000350636300087