Composite yarns with antibacterial nanofibrous sheaths produced by collectorless alternating-current electrospinning for suture applications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24210%2F22%3A00009032" target="_blank" >RIV/46747885:24210/22:00009032 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24410/22:00009032 RIV/46747885:24510/22:00009032 RIV/46747885:24620/22:00009032

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/app.51851?saml_referrer" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/app.51851?saml_referrer</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/app.51851" target="_blank" >10.1002/app.51851</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Composite yarns with antibacterial nanofibrous sheaths produced by collectorless alternating-current electrospinning for suture applications

Popis výsledku v původním jazyce

In this work, PU- and PA6-based CNYs were prepared using a novel collectorless ACES approach. CHX was added to the pristine PU and PA6 spinning solutions to fabricate antibacterial CNYs which can be used in suture applications. SEM illustrated that PU nanofibers can be formed by ACES with a DMF-based solution, which further expands the polymer range for this method. For PA6, sulfuric acid was used as an additive to the polymeric solution to improve the spinnability, similar to what was previously described. SEM images illustrated that the PU- and PA6-based electrospun nanofibrous sheath was completely wound around the core yarn. After addition of CHX to the electrospinning solution, the obtained nanofibers in the sheath showed a more straightened morphology, which could be attributed to the molecular interactions of the CHX with the polymer chains. Nonetheless, both the pristine and CHX-containing CNYs had a clear envelope that was wound around the PA6 core yarn, indicating that the addition of CHX did not impair the electrospinning process. The presence of CHX in the PU-CHX and PA6-CHX CNYs was confirmed by the HPLC of solutions obtained from the aforementioned yarns. The amount of incorporated CHX was higher for the PA6-based CNYs than for the PU-based CNYs, which might be associated with the lower polymer concentration in the former electrospinning solution. XPS and ATR-FTIR, however, indicated that the amount of incorporated antibacterial agent was relatively low in the PU- and PA6-based CNYs, as the Cl-amount was undetectable with the former technique, while only small peak shifts were obtained in the latter. Linear density measurements and mechanical testing showed that CHX addition resulted in a lower linear density and envelope adhesion force for both polymers. Additionally, it was observed that a higher linear density of the envelope resulted in a higher envelope adhesion force. The PU CNYs also had higher tensile strength than the PA6 CNYs, while the CHX addition did not result in a significant change in this property. Moreover, all the CNYs outperformed the PA6 core yarn in tensile strength, indicating that the proposed ACES process is interesting to fabricate stronger sutures. TGA analysis indicated that all fabricated CNYs were thermally stable until at least 250°C, which is definitely sufficient for in vivo use as surgical sutures. Subsequently, a MTT assay showed that the PU, PA6, and PA-CHX CNYs were biocompatible, while the PU-CHX CNYs were cytotoxic, probably because of the higher amount of CHX that leaches out of these yarns. Last, antibacterial tests showed that the PA6-CHX CNYs inhibited S. aureus and E. coli bacterial growth, while the PU-CHX CNY did not show any antibacterial effect, indicating that the PA6-CHX CNYs can be used as antibacterial, biocompatible and mechanically performant surgical sutures.

Název v anglickém jazyce

Composite yarns with antibacterial nanofibrous sheaths produced by collectorless alternating-current electrospinning for suture applications

Popis výsledku anglicky

In this work, PU- and PA6-based CNYs were prepared using a novel collectorless ACES approach. CHX was added to the pristine PU and PA6 spinning solutions to fabricate antibacterial CNYs which can be used in suture applications. SEM illustrated that PU nanofibers can be formed by ACES with a DMF-based solution, which further expands the polymer range for this method. For PA6, sulfuric acid was used as an additive to the polymeric solution to improve the spinnability, similar to what was previously described. SEM images illustrated that the PU- and PA6-based electrospun nanofibrous sheath was completely wound around the core yarn. After addition of CHX to the electrospinning solution, the obtained nanofibers in the sheath showed a more straightened morphology, which could be attributed to the molecular interactions of the CHX with the polymer chains. Nonetheless, both the pristine and CHX-containing CNYs had a clear envelope that was wound around the PA6 core yarn, indicating that the addition of CHX did not impair the electrospinning process. The presence of CHX in the PU-CHX and PA6-CHX CNYs was confirmed by the HPLC of solutions obtained from the aforementioned yarns. The amount of incorporated CHX was higher for the PA6-based CNYs than for the PU-based CNYs, which might be associated with the lower polymer concentration in the former electrospinning solution. XPS and ATR-FTIR, however, indicated that the amount of incorporated antibacterial agent was relatively low in the PU- and PA6-based CNYs, as the Cl-amount was undetectable with the former technique, while only small peak shifts were obtained in the latter. Linear density measurements and mechanical testing showed that CHX addition resulted in a lower linear density and envelope adhesion force for both polymers. Additionally, it was observed that a higher linear density of the envelope resulted in a higher envelope adhesion force. The PU CNYs also had higher tensile strength than the PA6 CNYs, while the CHX addition did not result in a significant change in this property. Moreover, all the CNYs outperformed the PA6 core yarn in tensile strength, indicating that the proposed ACES process is interesting to fabricate stronger sutures. TGA analysis indicated that all fabricated CNYs were thermally stable until at least 250°C, which is definitely sufficient for in vivo use as surgical sutures. Subsequently, a MTT assay showed that the PU, PA6, and PA-CHX CNYs were biocompatible, while the PU-CHX CNYs were cytotoxic, probably because of the higher amount of CHX that leaches out of these yarns. Last, antibacterial tests showed that the PA6-CHX CNYs inhibited S. aureus and E. coli bacterial growth, while the PU-CHX CNY did not show any antibacterial effect, indicating that the PA6-CHX CNYs can be used as antibacterial, biocompatible and mechanically performant surgical sutures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Polymer Science

ISSN

0021-8995

e-ISSN

—

Svazek periodika

139

Číslo periodika v rámci svazku

13

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

—

Kód UT WoS článku

000715580100001

EID výsledku v databázi Scopus

2-s2.0-85118596026