Suitability of Lyophilized Polymers based on CMC for Cell Proliferation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24410%2F15%3A00002536" target="_blank" >RIV/46747885:24410/15:00002536 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24620/15:00002536

Výsledek na webu

<a href="http://www.nanocon.eu/cz/" target="_blank" >http://www.nanocon.eu/cz/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Suitability of Lyophilized Polymers based on CMC for Cell Proliferation

Popis výsledku v původním jazyce

The aim of the study was to develop a material suitable for wound dressing which would be also suitable for cell growth and cell proliferation. Such matrix should have the structure of interconnected pores and a high porosity to ensure the adequate penetration of the cells and the supply of nutrients to the cells during their formation, diffusion of waste products out of the matrix and exclusion the products of matrix degradation. The study is focused on optimization the method of preparation of 3D porous structures and subsequent testing of cytotoxicity of thus prepared structures. The cytotoxicity of the structers was tested on human skin fibroblast cells. The cytotoxicity was tested by measuring the cell viability and using XTT assay. Another performed test was the test of biocidity/biodegradability. XTT assay results demonstrated that the lyophilized sample of carboxymethylcellulose is suitable for cell proliferation. Better cell proliferation is also caused by the increase of porosity made by lyophilization.

Název v anglickém jazyce

Suitability of Lyophilized Polymers based on CMC for Cell Proliferation

Popis výsledku anglicky

The aim of the study was to develop a material suitable for wound dressing which would be also suitable for cell growth and cell proliferation. Such matrix should have the structure of interconnected pores and a high porosity to ensure the adequate penetration of the cells and the supply of nutrients to the cells during their formation, diffusion of waste products out of the matrix and exclusion the products of matrix degradation. The study is focused on optimization the method of preparation of 3D porous structures and subsequent testing of cytotoxicity of thus prepared structures. The cytotoxicity of the structers was tested on human skin fibroblast cells. The cytotoxicity was tested by measuring the cell viability and using XTT assay. Another performed test was the test of biocidity/biodegradability. XTT assay results demonstrated that the lyophilized sample of carboxymethylcellulose is suitable for cell proliferation. Better cell proliferation is also caused by the increase of porosity made by lyophilization.

Druh

D - Stať ve sborníku

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2015: 7TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION

ISBN

978-80-87294-63-5

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

565-570

Název nakladatele

Tanger Ltd. Ostrava

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

1. 1. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000374708800099