Comparison study of nanofibers, composite nano/microfiber materials, molecularly imprinted polymers, and core-shell sorbents used for on-line extraction-liquid chromatography of ochratoxins in Tokaj wines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24410%2F21%3A00009227" target="_blank" >RIV/46747885:24410/21:00009227 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/21:10430858

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X21007669?via=ihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X21007669?via=ihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2021.106680" target="_blank" >10.1016/j.microc.2021.106680</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison study of nanofibers, composite nano/microfiber materials, molecularly imprinted polymers, and core-shell sorbents used for on-line extraction-liquid chromatography of ochratoxins in Tokaj wines

Popis výsledku v původním jazyce

On-line solid phase extraction coupled with liquid chromatography enables high sample throughput, preconcentration of analytes, efficient clean-up, and separation in a single step. Our comparison study focused on the selection of an optimal SPE sorbent for on-line extraction of ochratoxins A and B allowing fast and sensitive control of mycotoxin contamination. Four types of fibrous sorbents including polyethylene microfibers, polypropylene microfibers, polycaprolactone microfibers/nanofibers composite, and polycaprolactone microfibers/polyvinylidene difluoride nanofibers composite together with selective molecularly imprinted polymers, and conventional C18 core-shell particle sorbent were tested, and efficiency of extraction was compared in on-line mode. Clean-up efficiency and ability to remove matrix interferences with the subsequent chromatographic separation were optimized for all extraction materials followed by validation of the methods. Both nanofibers and molecularly imprinted polymer sorbents were packed in a stainless-steel cartridge and directly connected to the HPLC system. Only polyethylene microfibers among all the tested fibers achieved the selectivity desired for resolution of ochratoxins from the wine matrix with the validation parameters comparable to commercial C18 sorbent. The best results featuring a limit of detection 0.03–0.06 µg L⁻¹, recoveries 92-97% with RSD b2%, and only 6 min overall analysis time were obtained after extraction using C18 particles. Features including stability of fibers in organic solvents, under high pressure, and their multiple reusability were also proven. Comparison of several types of extraction sorbents for ochratoxins was demonstrated with their on-line extraction followed by liquid chromatography with fluorescence determination using Tokaj wine as a complex matrix.

Název v anglickém jazyce

Comparison study of nanofibers, composite nano/microfiber materials, molecularly imprinted polymers, and core-shell sorbents used for on-line extraction-liquid chromatography of ochratoxins in Tokaj wines

Popis výsledku anglicky

On-line solid phase extraction coupled with liquid chromatography enables high sample throughput, preconcentration of analytes, efficient clean-up, and separation in a single step. Our comparison study focused on the selection of an optimal SPE sorbent for on-line extraction of ochratoxins A and B allowing fast and sensitive control of mycotoxin contamination. Four types of fibrous sorbents including polyethylene microfibers, polypropylene microfibers, polycaprolactone microfibers/nanofibers composite, and polycaprolactone microfibers/polyvinylidene difluoride nanofibers composite together with selective molecularly imprinted polymers, and conventional C18 core-shell particle sorbent were tested, and efficiency of extraction was compared in on-line mode. Clean-up efficiency and ability to remove matrix interferences with the subsequent chromatographic separation were optimized for all extraction materials followed by validation of the methods. Both nanofibers and molecularly imprinted polymer sorbents were packed in a stainless-steel cartridge and directly connected to the HPLC system. Only polyethylene microfibers among all the tested fibers achieved the selectivity desired for resolution of ochratoxins from the wine matrix with the validation parameters comparable to commercial C18 sorbent. The best results featuring a limit of detection 0.03–0.06 µg L⁻¹, recoveries 92-97% with RSD b2%, and only 6 min overall analysis time were obtained after extraction using C18 particles. Features including stability of fibers in organic solvents, under high pressure, and their multiple reusability were also proven. Comparison of several types of extraction sorbents for ochratoxins was demonstrated with their on-line extraction followed by liquid chromatography with fluorescence determination using Tokaj wine as a complex matrix.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

—

Svazek periodika

170

Číslo periodika v rámci svazku

106680

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000697190000007

EID výsledku v databázi Scopus

2-s2.0-85111849596