Assessing the Impact of Polyamide Nanofibrous Material Areal Weight on Lacticaseibacillus rhamnosus Biofilm Formation and Resistance to Storage Conditions and Contamination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24510%2F25%3A00014357" target="_blank" >RIV/46747885:24510/25:00014357 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/25:43932741 RIV/60461373:22340/25:43932741

Výsledek na webu

<a href="https://pubs.acs.org/doi/full/10.1021/acsomega.5c01042" target="_blank" >https://pubs.acs.org/doi/full/10.1021/acsomega.5c01042</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsomega.5c01042" target="_blank" >10.1021/acsomega.5c01042</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessing the Impact of Polyamide Nanofibrous Material Areal Weight on Lacticaseibacillus rhamnosus Biofilm Formation and Resistance to Storage Conditions and Contamination

Popis výsledku v původním jazyce

Probiotic biofilms are considered the fourth most advanced generation of probiotics. To maximize the benefits of probiotic biofilms, suitable carriers ensuring bacterial viability during storage are being sought. The use of nanofibrous platforms is beginning to appear as one of the most promising approaches. We investigated the influence of three polyamide (PA) nanofibrous materials with different areal weights (5, 11, 27 g/m2) and the resulting morphological properties on the biofilm formation of Lacticaseibacillus rhamnosus ATCC 9595 and its tolerance to various conditions. PA promoted biofilm formation more than the reference material, polystyrene. PA‘s areal weight influenced the biofilm biomass amount, phenotype, and structure; PAs with a high areal weight promoted biofilm formation. Further, we examined the tolerance of matured biofilms on the PAs to various external conditions: (i) storage temperature (-20, 4, 21 degrees C), environment (aqueous/dry), and time (0-35 days), (ii) pH (2, 4, 6, 7, 8 and 10), and (iii) bacterial contamination by Staphylococcus aureus and Escherichia coli. Generally, PAs increased biofilm resistance, and the areal weight of the PA played a crucial role in it. The PA with the highest areal weight (27 g/m2) provided the highest long-term stability and tolerance of the biofilm and thus was confirmed to be the most suitable tested nanomaterial. The overall results suggest that the presented PAs could be suitable carriers of probiotic biofilm, enabling large-scale production. We also highlight the need for further research on the influence of nanomaterials‘ morphology on microbial interactions, possibly enabling target modification for a particular use.

Název v anglickém jazyce

Assessing the Impact of Polyamide Nanofibrous Material Areal Weight on Lacticaseibacillus rhamnosus Biofilm Formation and Resistance to Storage Conditions and Contamination

Popis výsledku anglicky

Probiotic biofilms are considered the fourth most advanced generation of probiotics. To maximize the benefits of probiotic biofilms, suitable carriers ensuring bacterial viability during storage are being sought. The use of nanofibrous platforms is beginning to appear as one of the most promising approaches. We investigated the influence of three polyamide (PA) nanofibrous materials with different areal weights (5, 11, 27 g/m2) and the resulting morphological properties on the biofilm formation of Lacticaseibacillus rhamnosus ATCC 9595 and its tolerance to various conditions. PA promoted biofilm formation more than the reference material, polystyrene. PA‘s areal weight influenced the biofilm biomass amount, phenotype, and structure; PAs with a high areal weight promoted biofilm formation. Further, we examined the tolerance of matured biofilms on the PAs to various external conditions: (i) storage temperature (-20, 4, 21 degrees C), environment (aqueous/dry), and time (0-35 days), (ii) pH (2, 4, 6, 7, 8 and 10), and (iii) bacterial contamination by Staphylococcus aureus and Escherichia coli. Generally, PAs increased biofilm resistance, and the areal weight of the PA played a crucial role in it. The PA with the highest areal weight (27 g/m2) provided the highest long-term stability and tolerance of the biofilm and thus was confirmed to be the most suitable tested nanomaterial. The overall results suggest that the presented PAs could be suitable carriers of probiotic biofilm, enabling large-scale production. We also highlight the need for further research on the influence of nanomaterials‘ morphology on microbial interactions, possibly enabling target modification for a particular use.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10400 - Chemical sciences

Projekt

<a href="/cs/project/GA23-05154S" target="_blank" >GA23-05154S: Interakce prokaryotických a eukaryotických buněk s nanovlákny s různou morfologií a strukturou</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS OMEGA>

ISSN

2470-1343

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

41

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

47867-47878

Kód UT WoS článku

001588882300001

EID výsledku v databázi Scopus

2-s2.0-105019095732