Graphene monolayer as an appropriate substrate for mesenchymal stem cells support in regenerative medicine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24530%2F23%3A00012155" target="_blank" >RIV/46747885:24530/23:00012155 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/23:10465974

Výsledek na webu

<a href="https://or.niscpr.res.in/index.php/IJEB/article/view/174/1" target="_blank" >https://or.niscpr.res.in/index.php/IJEB/article/view/174/1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.56042/ijeb.v61i04.174" target="_blank" >10.56042/ijeb.v61i04.174</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Graphene monolayer as an appropriate substrate for mesenchymal stem cells support in regenerative medicine

Popis výsledku v původním jazyce

Synergistic effect of mesenchymal stem cells and graphene monolayer is an undiscovered topic and crucial in the context of creating a graphene skin dressing. Moreover, capturing early changes in the cytoskeleton may prove to be a valuable indicator of cell condition after direct contact with the biomaterial. In this context, here, we investigated the response of human bone marrow mesenchymal stem cells (hBM-MSCs) to graphene monolayer as a scaffold, based on cytoskeleton architecture. Three main components of the cytoskeleton — microfilaments, intermediate filaments and microtubules were characterized as well as analyzed its entire cell morphology (focal adhesions). Based on microscopic analysis and cell area measurements, it can be stated that graphene did not cause a damage or disturbance of any cytoskeleton members and linker proteins (plectin, vinculin) and gap junction protein (connexin 43). hBM-MSCs growing on the graphene monolayer were more spread than cells growing on the glass but without any other significant difference. This suggested that graphene substrate as a monolayer could be a structural reinforcement for MSCs cultivation without affecting their cytoskeleton, which determines cell integrity, connections and migration, all important cell properties in the process of skin wound healing.

Název v anglickém jazyce

Graphene monolayer as an appropriate substrate for mesenchymal stem cells support in regenerative medicine

Popis výsledku anglicky

Synergistic effect of mesenchymal stem cells and graphene monolayer is an undiscovered topic and crucial in the context of creating a graphene skin dressing. Moreover, capturing early changes in the cytoskeleton may prove to be a valuable indicator of cell condition after direct contact with the biomaterial. In this context, here, we investigated the response of human bone marrow mesenchymal stem cells (hBM-MSCs) to graphene monolayer as a scaffold, based on cytoskeleton architecture. Three main components of the cytoskeleton — microfilaments, intermediate filaments and microtubules were characterized as well as analyzed its entire cell morphology (focal adhesions). Based on microscopic analysis and cell area measurements, it can be stated that graphene did not cause a damage or disturbance of any cytoskeleton members and linker proteins (plectin, vinculin) and gap junction protein (connexin 43). hBM-MSCs growing on the graphene monolayer were more spread than cells growing on the glass but without any other significant difference. This suggested that graphene substrate as a monolayer could be a structural reinforcement for MSCs cultivation without affecting their cytoskeleton, which determines cell integrity, connections and migration, all important cell properties in the process of skin wound healing.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Indian Journal of Experimental Biology

ISSN

0019-5189

e-ISSN

—

Svazek periodika

61

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

9

Strana od-do

235-243

Kód UT WoS článku

000996067000001

EID výsledku v databázi Scopus

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