Comparison of the Influence of Nanoparticles on Escherichia Coli and Pseudomonas Aeruginosa Bacterial Populations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F15%3A00000073" target="_blank" >RIV/46747885:24620/15:00000073 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24220/15:#0003354

Výsledek na webu

<a href="http://www.nanocon.eu/files/proceedings/20/reports/3417.pdf" target="_blank" >http://www.nanocon.eu/files/proceedings/20/reports/3417.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of the Influence of Nanoparticles on Escherichia Coli and Pseudomonas Aeruginosa Bacterial Populations

Popis výsledku v původním jazyce

As use of nanoparticles continues to increase in various fields of human activity, it becomes increasingly important to understand all interactions that occur between nanoparticles and cells. In this experiment, we explore the influence of several types of nanoparticle on populations of facultative anaerobic bacteria (Escherichia coli) and aerobic bacteria (Pseudomonas aeruginosa). Nanoparticle toxicity (nanoparticle concentration 1 g/l) was evaluated through respirometry and microscopic fluorescence analysis, which allows for observation and comparison of both living and dead cells in a sample. These two methods were supplemented by determination of colony forming units. Basal salt medium (BSM) with differing concentration of glucose and a soya broth medium were used for both bacterial populations as a culture medium. Nanodiamond proved to be the least toxic of the nanoparticles tested (though these contained impurities that may have affected the results), while nanoparticles of praseodymium had the most toxic effect on bacterial populations. A scanning electron microscope (SEM) was used to assess both the appearance and the approximate sizes of the nanoparticles and bacteria. The bacteria clearly differed in size, with E. coli averaging 3-4 µm and P. aeruginosa averaging 1.5 µm. The various nanoparticles ranged between 50 and 200 nm. SEM confirmed colonization of nanoparticle aggregate surfaces by bacteria in almost all samples

Název v anglickém jazyce

Comparison of the Influence of Nanoparticles on Escherichia Coli and Pseudomonas Aeruginosa Bacterial Populations

Popis výsledku anglicky

As use of nanoparticles continues to increase in various fields of human activity, it becomes increasingly important to understand all interactions that occur between nanoparticles and cells. In this experiment, we explore the influence of several types of nanoparticle on populations of facultative anaerobic bacteria (Escherichia coli) and aerobic bacteria (Pseudomonas aeruginosa). Nanoparticle toxicity (nanoparticle concentration 1 g/l) was evaluated through respirometry and microscopic fluorescence analysis, which allows for observation and comparison of both living and dead cells in a sample. These two methods were supplemented by determination of colony forming units. Basal salt medium (BSM) with differing concentration of glucose and a soya broth medium were used for both bacterial populations as a culture medium. Nanodiamond proved to be the least toxic of the nanoparticles tested (though these contained impurities that may have affected the results), while nanoparticles of praseodymium had the most toxic effect on bacterial populations. A scanning electron microscope (SEM) was used to assess both the appearance and the approximate sizes of the nanoparticles and bacteria. The bacteria clearly differed in size, with E. coli averaging 3-4 µm and P. aeruginosa averaging 1.5 µm. The various nanoparticles ranged between 50 and 200 nm. SEM confirmed colonization of nanoparticle aggregate surfaces by bacteria in almost all samples

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NanoCon 2014, 6th International Conference, Conference Proceedings

ISBN

978-80-87294-53-6

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

707-711

Název nakladatele

TANGER Ltd

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

1. 1. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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