Development of nanofiber carriers for monitoring of biomass at a contaminated site

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F17%3A00004414" target="_blank" >RIV/46747885:24620/17:00004414 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24620/17:00004417

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of nanofiber carriers for monitoring of biomass at a contaminated site

Popis výsledku v původním jazyce

Introduction: Biomass sampling is a crucial factor in understanding and controlling remediation processes ongoing at contaminated sites. There are several methods and instruments used for sampling. However, main disadvantages as time consumption, difficult usage and need to prepare sample prior to analysis remain. Objectives: Goal of this study was, therefore, to develop new biomass carriers with specific properties to overcome mentioned limitations. As nanofibers have very high specific surface area they were used for preparation of biomass carriers suitable for monitoring of biomass. Material and methods: Carriers made of supporting thread coated by nanofiber layer varied in shape (2) and nanofiber surface density (3 dtex and 10 dtex). Carriers without nanofibers were also tested as reference sample. All carriers were submerged into 3 wells with different contamination and long-termly monitored. Two wells were contaminated by chlorinated hydrocarbons, third one by BTEX. Carriers were sampled in month interval for one year. Groundwater was also taken together with carriers. DNA was isolated by FastDNA SPIN Kit for Soil according to manufacturer`s protocol. DNA concentration was measured on Qubit 2.0 fluorometer. Extracted DNA was used as a template for real-time PCR amplification. 16S rDNA gene was used as a total bacterial community marker. Specific genes were used for detection of ongoing dehalogenation (vcrA) or BTEX degradation (catechol 2,3-dioxygenase gene). Results: After 3 months, real-time PCR values detected on carriers were approximately same compared to groundwater samples. Only small variances in the values between individual carriers were observed, thus indicating stability of attached biomass. Although shape of carriers did not show clear impact on biomass growth, growth on nanofiber carriers was slightly higher compared to reference carriers in well contaminated by BTEX. In the wells with chlorinated hydrocarbons, impact of nanofiber density on growth of biomass was not clearly proven. Conclusions: Results showed that developed nanofiber carriers are promising tool for long-term monitoring at contaminated sites as providing stable biomass sample and response. Moreover, they are very easy to use and no sample preparation is required prior to analysis.

Název v anglickém jazyce

Development of nanofiber carriers for monitoring of biomass at a contaminated site

Popis výsledku anglicky

Introduction: Biomass sampling is a crucial factor in understanding and controlling remediation processes ongoing at contaminated sites. There are several methods and instruments used for sampling. However, main disadvantages as time consumption, difficult usage and need to prepare sample prior to analysis remain. Objectives: Goal of this study was, therefore, to develop new biomass carriers with specific properties to overcome mentioned limitations. As nanofibers have very high specific surface area they were used for preparation of biomass carriers suitable for monitoring of biomass. Material and methods: Carriers made of supporting thread coated by nanofiber layer varied in shape (2) and nanofiber surface density (3 dtex and 10 dtex). Carriers without nanofibers were also tested as reference sample. All carriers were submerged into 3 wells with different contamination and long-termly monitored. Two wells were contaminated by chlorinated hydrocarbons, third one by BTEX. Carriers were sampled in month interval for one year. Groundwater was also taken together with carriers. DNA was isolated by FastDNA SPIN Kit for Soil according to manufacturer`s protocol. DNA concentration was measured on Qubit 2.0 fluorometer. Extracted DNA was used as a template for real-time PCR amplification. 16S rDNA gene was used as a total bacterial community marker. Specific genes were used for detection of ongoing dehalogenation (vcrA) or BTEX degradation (catechol 2,3-dioxygenase gene). Results: After 3 months, real-time PCR values detected on carriers were approximately same compared to groundwater samples. Only small variances in the values between individual carriers were observed, thus indicating stability of attached biomass. Although shape of carriers did not show clear impact on biomass growth, growth on nanofiber carriers was slightly higher compared to reference carriers in well contaminated by BTEX. In the wells with chlorinated hydrocarbons, impact of nanofiber density on growth of biomass was not clearly proven. Conclusions: Results showed that developed nanofiber carriers are promising tool for long-term monitoring at contaminated sites as providing stable biomass sample and response. Moreover, they are very easy to use and no sample preparation is required prior to analysis.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.