Application of laser-induced breakdown cavitation bubbles for cell lysis in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F19%3A00006990" target="_blank" >RIV/46747885:24620/19:00006990 - isvavai.cz</a>

Výsledek na webu

<a href="https://innovareacademics.in/journals/index.php/ijap/article/view/35860" target="_blank" >https://innovareacademics.in/journals/index.php/ijap/article/view/35860</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.22159/ijap.2019.v11s5.T3059" target="_blank" >10.22159/ijap.2019.v11s5.T3059</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of laser-induced breakdown cavitation bubbles for cell lysis in vitro

Popis výsledku v původním jazyce

Objective: Understanding the basic mechanism of the cavitation bubble action on living cells as a crucial step of development and application of sophisticated methods based on controlled cavitation in cell behaviour manipulation. Optimisation of parameters in order to expand cell lysis region created by a single bubble. Methods: The cavitation bubbles are generated by the laser-induced breakdown method. The impact of controlled cavitation bubble on the biological system is synchronously monitored under a microscope and recorded. Visualization of the cavitation bubble course is monitored by a high-speed camera. The impact of technology on the healthy confluent cell layer is verified. Evaluation of the cavitation bubbles` effect on cells in real time and by subsequent analysis of the cell lysis region and impact of the cavitation bubble on cell viability is carried out by optical visualization and life/ dead fluorescence staining. Results: Cavitation bubble induced in distance of 1.5 mm from the cell surface overcomes properties of sessile bubble and enables to create cell lysis region over 1000 µm in diameter due to transient shear stress produced by liquid displaced by the bubble expansion. Conclusion: Cell lysis region is strongly dependent on the spot laser energy (SLE) and the bubble induction distance from cells. This knowledge is crucial for application in chemical free cell lysis in vitro, wound induction for experimental purposes and cell layers patterning in desired scale.

Název v anglickém jazyce

Application of laser-induced breakdown cavitation bubbles for cell lysis in vitro

Popis výsledku anglicky

Objective: Understanding the basic mechanism of the cavitation bubble action on living cells as a crucial step of development and application of sophisticated methods based on controlled cavitation in cell behaviour manipulation. Optimisation of parameters in order to expand cell lysis region created by a single bubble. Methods: The cavitation bubbles are generated by the laser-induced breakdown method. The impact of controlled cavitation bubble on the biological system is synchronously monitored under a microscope and recorded. Visualization of the cavitation bubble course is monitored by a high-speed camera. The impact of technology on the healthy confluent cell layer is verified. Evaluation of the cavitation bubbles` effect on cells in real time and by subsequent analysis of the cell lysis region and impact of the cavitation bubble on cell viability is carried out by optical visualization and life/ dead fluorescence staining. Results: Cavitation bubble induced in distance of 1.5 mm from the cell surface overcomes properties of sessile bubble and enables to create cell lysis region over 1000 µm in diameter due to transient shear stress produced by liquid displaced by the bubble expansion. Conclusion: Cell lysis region is strongly dependent on the spot laser energy (SLE) and the bubble induction distance from cells. This knowledge is crucial for application in chemical free cell lysis in vitro, wound induction for experimental purposes and cell layers patterning in desired scale.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/EF16_019%2F0000843" target="_blank" >EF16_019/0000843: Hybridní materiály pro hierarchické struktury</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Applied Pharmaceutics

ISSN

09757058

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

5

Strana od-do

186-190

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85073527866