Sterilization of Nanofiber Biomass Carriers for Biotechnology Applications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F19%3A00007202" target="_blank" >RIV/46747885:24620/19:00007202 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sterilization of Nanofiber Biomass Carriers for Biotechnology Applications

Popis výsledku v původním jazyce

In the last decade, nanomaterials have gained a lot of attention as providing new options and potentials in many areas. Since nanofibers have a very high specific surface area, they are promising tool applicable in various fields, particularly in biomedical and environmental applications. For most of such applications, sterility of nanofibers is a critical factor to avoid possible negative contamination. For example, the nanofiber carriers used for the colonization of pure microbial culture have to be microorganism-free, and its structure must not be affected after the sterilization. In this study, nanofiber biomass carriers were sterilized using 3 various physical and 4 chemical methods. Physical methods implied steam, ultraviolet irradiation and ultrasound. Chemical methods were as follow: absolute ethanol, a solution of chlorine dioxide, hydrogen peroxide and ozone. Two types of the nanofiber layer based on polyurethane, which showed in previous experiments very good biocompatibility, were prepared by the needleless electrospinning from a free liquid surface. DC and AC high voltage source were used for nanofibers preparation. The impact of sterilization method on the nanofiber layer was examined by optical and scanning electron microscopy. The efficiency of sterilization was assessed by cultivation methods on the plate count agars and molecular-genetic methods, namely using a qPCR analysis. Nanofiber carriers sterilized by steam exhibited the highest efficiency of sterilization. The most significant damage of the nanofiber layer on the support thread was observed after the sterilization by steam and ozone. Results of cultivation and molecular-genetic methods also indicated that both nanofibers prepared using DC as well as AC electrospinning are partly antimicrobial already after their production. It was probably caused by the reagents used for the polymerization of polyurethane during its production. This effect will be subjected to chemical analysis in detail to detect potential antimicrobial agents. Further sterilization methods will be also texted in order to find the most efficient method or rather combination of methods, including the ambient conditions.

Název v anglickém jazyce

Sterilization of Nanofiber Biomass Carriers for Biotechnology Applications

Popis výsledku anglicky

In the last decade, nanomaterials have gained a lot of attention as providing new options and potentials in many areas. Since nanofibers have a very high specific surface area, they are promising tool applicable in various fields, particularly in biomedical and environmental applications. For most of such applications, sterility of nanofibers is a critical factor to avoid possible negative contamination. For example, the nanofiber carriers used for the colonization of pure microbial culture have to be microorganism-free, and its structure must not be affected after the sterilization. In this study, nanofiber biomass carriers were sterilized using 3 various physical and 4 chemical methods. Physical methods implied steam, ultraviolet irradiation and ultrasound. Chemical methods were as follow: absolute ethanol, a solution of chlorine dioxide, hydrogen peroxide and ozone. Two types of the nanofiber layer based on polyurethane, which showed in previous experiments very good biocompatibility, were prepared by the needleless electrospinning from a free liquid surface. DC and AC high voltage source were used for nanofibers preparation. The impact of sterilization method on the nanofiber layer was examined by optical and scanning electron microscopy. The efficiency of sterilization was assessed by cultivation methods on the plate count agars and molecular-genetic methods, namely using a qPCR analysis. Nanofiber carriers sterilized by steam exhibited the highest efficiency of sterilization. The most significant damage of the nanofiber layer on the support thread was observed after the sterilization by steam and ozone. Results of cultivation and molecular-genetic methods also indicated that both nanofibers prepared using DC as well as AC electrospinning are partly antimicrobial already after their production. It was probably caused by the reagents used for the polymerization of polyurethane during its production. This effect will be subjected to chemical analysis in detail to detect potential antimicrobial agents. Further sterilization methods will be also texted in order to find the most efficient method or rather combination of methods, including the ambient conditions.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/TH03030333" target="_blank" >TH03030333: Technologie mikrobiologicky čistých povrchů nanotechnologických materiálů pro biotechnologické aplikace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů