Chapter 4 - Molecular techniques for understanding microbial abundance and activity in clay barriers used for geodisposal

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F20%3A00008706" target="_blank" >RIV/46747885:24620/20:00008706 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/B978-0-12-818695-4.00004-6" target="_blank" >https://doi.org/10.1016/B978-0-12-818695-4.00004-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/B978-0-12-818695-4.00004-6" target="_blank" >10.1016/B978-0-12-818695-4.00004-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chapter 4 - Molecular techniques for understanding microbial abundance and activity in clay barriers used for geodisposal

Popis výsledku v původním jazyce

Clays are commonly used in design concepts for geological disposal of nuclear waste. It is thus essential to identify and quantify microbial communities in clay-rich samples to study microbial processes during geological disposal. Although advances in culture-independent techniques have enabled detailed studies of microbial communities in diverse ecosystems, the efficiency and sensitivity of these molecular techniques depend on characteristics of the environment studied. Moreover, the outcome of nucleic acid-based approaches depends on the extraction method, primer specificity, PCR amplification, sequencing artifacts, and downstream bioinformatic analyses. Clays are recalcitrant to DNA extraction and are challenging for analysis by standard techniques using viability stains and measurement of metabolic activity. This chapter explores the impact of various sequencing and bioinformatic pipelines used for 16S rRNA gene profiling of microbial communities and compares the efficiency of different DNA extraction methods from clay. Moreover, we also discuss non-DNA-based techniques used to assess microbial activity and viability in clay samples.

Název v anglickém jazyce

Chapter 4 - Molecular techniques for understanding microbial abundance and activity in clay barriers used for geodisposal

Popis výsledku anglicky

Clays are commonly used in design concepts for geological disposal of nuclear waste. It is thus essential to identify and quantify microbial communities in clay-rich samples to study microbial processes during geological disposal. Although advances in culture-independent techniques have enabled detailed studies of microbial communities in diverse ecosystems, the efficiency and sensitivity of these molecular techniques depend on characteristics of the environment studied. Moreover, the outcome of nucleic acid-based approaches depends on the extraction method, primer specificity, PCR amplification, sequencing artifacts, and downstream bioinformatic analyses. Clays are recalcitrant to DNA extraction and are challenging for analysis by standard techniques using viability stains and measurement of metabolic activity. This chapter explores the impact of various sequencing and bioinformatic pipelines used for 16S rRNA gene profiling of microbial communities and compares the efficiency of different DNA extraction methods from clay. Moreover, we also discuss non-DNA-based techniques used to assess microbial activity and viability in clay samples.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

The Microbiology of Nuclear Waste Disposal

ISBN

978-0-12-818695-4

Počet stran výsledku

26

Strana od-do

71-96

Počet stran knihy

355

Název nakladatele

Elsevier

Místo vydání

—

Kód UT WoS kapitoly

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