Haploidní zygotická embryogeneze indukovaná pylem kukuřice a androgeneze in vitro u českých šlechtitelských genotypů pšenice

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46904841%3A_____%2F01%3A00010293" target="_blank" >RIV/46904841:_____/01:00010293 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro haploid zygotic embryogenesis due to pollination with maize pollen and induced in vitro androgenesis in Czech wheat breeding genotypes

Popis výsledku v původním jazyce

Induction of haploid zygotic embryogenesis and androgenesis was studied in 72 and 31 wheat genotypes, respectively. Green zygotic plants (haploids only) were obtained in 52 genotypes (72 %), and green androgenic regenerants (haploids and spontaneous polyhaploids) were observed in 17 genotypes (55 %). In zygotic embryogenesis embryos developed in 65 genotypes (90%), and in androgenesis embryos or microsporial calli were detected in 28 genotypes (90 %). The highest frequency of haploid zygotic regenerantswas 0.24 per pollinated flower, whilst that of androgenic regenerants was 0.08 per cultured anther (0.24 per three anthers in one flower). On average, in induced androgenesis the plants were produced by one out of three androgenic anthers. In induced haploid zygotic embryogenesis the plants were regenerated by every third flower (ovary) with induced embryos. In a set of selected genotypes the yield of induced haploid zygotic embryogenesis was compared with induced pollen embryogenesis.

Název v anglickém jazyce

In vitro haploid zygotic embryogenesis due to pollination with maize pollen and induced in vitro androgenesis in Czech wheat breeding genotypes

Popis výsledku anglicky

Induction of haploid zygotic embryogenesis and androgenesis was studied in 72 and 31 wheat genotypes, respectively. Green zygotic plants (haploids only) were obtained in 52 genotypes (72 %), and green androgenic regenerants (haploids and spontaneous polyhaploids) were observed in 17 genotypes (55 %). In zygotic embryogenesis embryos developed in 65 genotypes (90%), and in androgenesis embryos or microsporial calli were detected in 28 genotypes (90 %). The highest frequency of haploid zygotic regenerantswas 0.24 per pollinated flower, whilst that of androgenic regenerants was 0.08 per cultured anther (0.24 per three anthers in one flower). On average, in induced androgenesis the plants were produced by one out of three androgenic anthers. In induced haploid zygotic embryogenesis the plants were regenerated by every third flower (ovary) with induced embryos. In a set of selected genotypes the yield of induced haploid zygotic embryogenesis was compared with induced pollen embryogenesis.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/GA521%2F96%2F0395" target="_blank" >GA521/96/0395: Indukce izogenních rostlin u šlechtitelských materiálů pšenice a ovsa pomocí zygotické embryogeneze po mezirodové hybridizaci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant, Soil and Environment

ISSN

0370-663X

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

193-200

Kód UT WoS článku

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EID výsledku v databázi Scopus

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