Použití izoenzymových analýz a fluorescentní mikroskopie pro detekci dihlaploiniho stavu rostlin u kmínu.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F48392952%3A_____%2F06%3AE0005395" target="_blank" >RIV/48392952:_____/06:E0005395 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of dihaploid status using isozyme analysis and fluorescent microscopy in caraway.

Popis výsledku v původním jazyce

Caraway (Carum carvi L.) is a traditional umbelliferous medicinal and cross-pollinated plant. Conventional breeding methods concentrated on high yield and quality of oil compounds of seeds are limited by low genetic variability (Nemeth 1998, Evenhuis etal. 1998). Current studies in Daucaceae family do not follow a possibility of dihaploid plants production trough in vitro pollen embryogenesis except carrot (Andersen et al. 1990, Adamus and Michalik 2003). The 10 selected genotypes of winter type caraway were tested for their ability to produce dihaploid plants (DH). The ploidy status of the regenerated was analysed using flow-cytometry. The beginning of switch pollen development from normal gametophytic to sporophytic pathway was investigated by methods of light and fluorescent microscopy with acetocarmine and DAPI staining, respectively. Donor, haploid, dihaploid an diploid plants were compared by esterase (EST) native PAGE analysis to determine of pollen/anther wall origin among the

Název v anglickém jazyce

Detection of dihaploid status using isozyme analysis and fluorescent microscopy in caraway.

Popis výsledku anglicky

Caraway (Carum carvi L.) is a traditional umbelliferous medicinal and cross-pollinated plant. Conventional breeding methods concentrated on high yield and quality of oil compounds of seeds are limited by low genetic variability (Nemeth 1998, Evenhuis etal. 1998). Current studies in Daucaceae family do not follow a possibility of dihaploid plants production trough in vitro pollen embryogenesis except carrot (Andersen et al. 1990, Adamus and Michalik 2003). The 10 selected genotypes of winter type caraway were tested for their ability to produce dihaploid plants (DH). The ploidy status of the regenerated was analysed using flow-cytometry. The beginning of switch pollen development from normal gametophytic to sporophytic pathway was investigated by methods of light and fluorescent microscopy with acetocarmine and DAPI staining, respectively. Donor, haploid, dihaploid an diploid plants were compared by esterase (EST) native PAGE analysis to determine of pollen/anther wall origin among the

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/QF4056" target="_blank" >QF4056: Využití stávajících odrůd kmínu kořenného (Carum carvi L.) a nových metod v jeho šlechtění pro zvýšení kvalitativních a kvantitativních parametrů.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů