Effect of the Ionizing Radiation Dose on Histone H2AX
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49777513%3A23220%2F21%3A43963762" target="_blank" >RIV/49777513:23220/21:43963762 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/49777513:23310/21:43963762
Výsledek na webu
<a href="https://www.djs.si/nene2021/proceedings/pdf/NENE2021_1015.pdf" target="_blank" >https://www.djs.si/nene2021/proceedings/pdf/NENE2021_1015.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of the Ionizing Radiation Dose on Histone H2AX
Popis výsledku v původním jazyce
In case the radiation dosimeters are not available, biological dosimetry represents an important method to estimate the absorbed dose of the exposed individuals during nuclear events. Nevertheless, the eukaryotic DNA is constantly exposed to exogenous and endogenous factors. Apart from the ionizing radiation, widescale DNA lesions are also induced by other harmful effects. DNA double-strand breaks (DSBs) are the gravest lesions. DSBs provoke an extensive reaction characterized by the expression of the H2AX molecule. The scope of this work is an assessment of a gamma radiation dose-effect on a human body in terms of expression of the H2AX in DNA. This contribution is focused on the expression scale of the phosphorylated H2AX molecule (g-H2AX), which highlights a DNA damage induced by the exposure to gamma radiation. The dependency between the share of g-H2AX molecule in an irradiated sample and the radiation dose was examined. The investigated subjects consist of fourteen samples of uncoagulable blood from healthy donors. The sample of each donor was divided into four test tubes – a negative control + three levels of gamma radiation (0.5 Gray, 1 Gray, 2 Gray). The irradiation was performed on a medical caesium source “Gammacell® 1000 Elite.” The evaluation was based on the method for determining g-H2AX after chemical stimulation DNA – extracorporeal photopheresis. The outcome of this work is the confirmation that the production of this molecule is dependent on the dose of gamma radiation. Owing to the g-H2AX characteristic, the finding of the relation between the share of g-H2AX molecule in a sample and a dose of radiation was statistically confirmed. Accordingly, the H2AX molecule can be considered a reliable specific marker for DNA damage. In the future, this method could find a purpose in practical events, for example, re-determination dose of radiation after nuclear events.
Název v anglickém jazyce
Effect of the Ionizing Radiation Dose on Histone H2AX
Popis výsledku anglicky
In case the radiation dosimeters are not available, biological dosimetry represents an important method to estimate the absorbed dose of the exposed individuals during nuclear events. Nevertheless, the eukaryotic DNA is constantly exposed to exogenous and endogenous factors. Apart from the ionizing radiation, widescale DNA lesions are also induced by other harmful effects. DNA double-strand breaks (DSBs) are the gravest lesions. DSBs provoke an extensive reaction characterized by the expression of the H2AX molecule. The scope of this work is an assessment of a gamma radiation dose-effect on a human body in terms of expression of the H2AX in DNA. This contribution is focused on the expression scale of the phosphorylated H2AX molecule (g-H2AX), which highlights a DNA damage induced by the exposure to gamma radiation. The dependency between the share of g-H2AX molecule in an irradiated sample and the radiation dose was examined. The investigated subjects consist of fourteen samples of uncoagulable blood from healthy donors. The sample of each donor was divided into four test tubes – a negative control + three levels of gamma radiation (0.5 Gray, 1 Gray, 2 Gray). The irradiation was performed on a medical caesium source “Gammacell® 1000 Elite.” The evaluation was based on the method for determining g-H2AX after chemical stimulation DNA – extracorporeal photopheresis. The outcome of this work is the confirmation that the production of this molecule is dependent on the dose of gamma radiation. Owing to the g-H2AX characteristic, the finding of the relation between the share of g-H2AX molecule in a sample and a dose of radiation was statistically confirmed. Accordingly, the H2AX molecule can be considered a reliable specific marker for DNA damage. In the future, this method could find a purpose in practical events, for example, re-determination dose of radiation after nuclear events.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Proceedings of the International Conference Nuclear Energy for New Europe (NENE 2021)
ISBN
978-961-6207-51-5
ISSN
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e-ISSN
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Počet stran výsledku
7
Strana od-do
"1015.1"-"1015.7"
Název nakladatele
Nuclear Society of Slovenia
Místo vydání
Ljubljana
Místo konání akce
on-line, Bled, Slovenia
Datum konání akce
6. 9. 2021
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
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