The histological microstructure and in vitro mechanical properties of the human female postmenopausal perineal body

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49777513%3A23520%2F19%3A43952172" target="_blank" >RIV/49777513:23520/19:43952172 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/19:10394119

Výsledek na webu

<a href="https://journals.lww.com/menopausejournal/Citation/2019/01000/The_histological_microstructure_and_in_vitro.10.aspx" target="_blank" >https://journals.lww.com/menopausejournal/Citation/2019/01000/The_histological_microstructure_and_in_vitro.10.aspx</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1097/GME.0000000000001166" target="_blank" >10.1097/GME.0000000000001166</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The histological microstructure and in vitro mechanical properties of the human female postmenopausal perineal body

Popis výsledku v původním jazyce

Objective: The perineal body connects muscles from the pelvic floor and is critical for support of the lower part of vagina and proper function of the anal canal. We determined mechanical parameters and volume fractions of main components of human female postmenopausal perineal body. Methods: The specimens were taken from 15 fresh female cadavers (age 74 +- 10, mean +- standard deviation). Seventy-five specimens from five regions of the perineal body were processed histologically to assess volume fractions of tissue components using stereological point testing grid. Fifteen specimens taken from the midline region were loaded uniaxially with 6 mm/min velocity until tissue rupture to determine Young’s modulus of elasticity, ultimate stresses, and strains. Results: The perineal body was composed of collagen (29%), adipose cells (27%), elastin (7%), smooth muscle (11%), and skeletal muscle (3%). The residual tissue (19%) constituted mostly peripheral nerves, lumina of blood vessels, fibroblasts, and fibrocytes. Young’s modulus of elasticity at midline region was 18 kPa (median) at small and 232 kPa at large deformations, respectively. The ultimate stress was 172 kPa and the ultimate strain was 1.4. Conclusions: We determined the structural and mechanical parameters of the perineal body. The resultant data could be used as input for models simulating pelvic floor prolapse or dysfunction.

Název v anglickém jazyce

The histological microstructure and in vitro mechanical properties of the human female postmenopausal perineal body

Popis výsledku anglicky

Objective: The perineal body connects muscles from the pelvic floor and is critical for support of the lower part of vagina and proper function of the anal canal. We determined mechanical parameters and volume fractions of main components of human female postmenopausal perineal body. Methods: The specimens were taken from 15 fresh female cadavers (age 74 +- 10, mean +- standard deviation). Seventy-five specimens from five regions of the perineal body were processed histologically to assess volume fractions of tissue components using stereological point testing grid. Fifteen specimens taken from the midline region were loaded uniaxially with 6 mm/min velocity until tissue rupture to determine Young’s modulus of elasticity, ultimate stresses, and strains. Results: The perineal body was composed of collagen (29%), adipose cells (27%), elastin (7%), smooth muscle (11%), and skeletal muscle (3%). The residual tissue (19%) constituted mostly peripheral nerves, lumina of blood vessels, fibroblasts, and fibrocytes. Young’s modulus of elasticity at midline region was 18 kPa (median) at small and 232 kPa at large deformations, respectively. The ultimate stress was 172 kPa and the ultimate strain was 1.4. Conclusions: We determined the structural and mechanical parameters of the perineal body. The resultant data could be used as input for models simulating pelvic floor prolapse or dysfunction.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20302 - Applied mechanics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY

ISSN

1072-3714

e-ISSN

—

Svazek periodika

26

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

66-77

Kód UT WoS článku

000467339900010

EID výsledku v databázi Scopus

2-s2.0-85059159584