Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12110%2F14%3A43887830" target="_blank" >RIV/60076658:12110/14:43887830 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3109/09553002.2014.887233" target="_blank" >http://dx.doi.org/10.3109/09553002.2014.887233</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3109/09553002.2014.887233" target="_blank" >10.3109/09553002.2014.887233</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell

Popis výsledku v původním jazyce

PURPOSE Low temperature (hypothermia) during irradiation leads to a reduced frequency of micronuclei in TK6 cells and it has been suggested that perturbation of cell cycle progression is responsible for this effect. The aim of the study was to test thishypothesis. MATERIALS AND METHODS Human lymphoblastoid TK6 cells were treated by a combination of hypothermia (0.8°C) and ionizing radiation in varying order (hypothermia before, during or after irradiation) and micronuclei were scored. Growth assay andtwo-dimensional flow cytometry was used to analyze cell cycle kinetics following irradiated of cells at 0.8°C or 37.0°C. RESULTS The temperature effect was observed at the level of micronuclei regardless of whether cells were cooled during or immediatelybefore or after the radiation exposure. No indication of cell cycle perturbation by combined exposure to hypothermia and radiation could be detected. CONCLUSIONS The protective effect of hypothermia observed at the level of cytogenetic d

Název v anglickém jazyce

Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cell

Popis výsledku anglicky

PURPOSE Low temperature (hypothermia) during irradiation leads to a reduced frequency of micronuclei in TK6 cells and it has been suggested that perturbation of cell cycle progression is responsible for this effect. The aim of the study was to test thishypothesis. MATERIALS AND METHODS Human lymphoblastoid TK6 cells were treated by a combination of hypothermia (0.8°C) and ionizing radiation in varying order (hypothermia before, during or after irradiation) and micronuclei were scored. Growth assay andtwo-dimensional flow cytometry was used to analyze cell cycle kinetics following irradiated of cells at 0.8°C or 37.0°C. RESULTS The temperature effect was observed at the level of micronuclei regardless of whether cells were cooled during or immediatelybefore or after the radiation exposure. No indication of cell cycle perturbation by combined exposure to hypothermia and radiation could be detected. CONCLUSIONS The protective effect of hypothermia observed at the level of cytogenetic d

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Radiation Biology

ISSN

0955-3002

e-ISSN

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Svazek periodika

90

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

318-324

Kód UT WoS článku

000333582100007

EID výsledku v databázi Scopus

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