Analysis of Genetic Damage in Lymphocytes of Former Uranium Processing Workers
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12110%2F15%3A43889944" target="_blank" >RIV/60076658:12110/15:43889944 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68378041:_____/15:00458998
Výsledek na webu
<a href="http://dx.doi.org/10.1159/000441889" target="_blank" >http://dx.doi.org/10.1159/000441889</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1159/000441889" target="_blank" >10.1159/000441889</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Analysis of Genetic Damage in Lymphocytes of Former Uranium Processing Workers
Popis výsledku v původním jazyce
The frequency of cells containing micronuclei (MN) and the presence of centromeres in these MN were analysed in lymphocytes of 98 men from Southern Bohemia. 46 of them had worked at the uranium processing plant, "MAPE Mydlovary", which has been defunct since 1991; 52 men were controls from the same area. Fluorescence in situ hybridization (FISH) method using Human Pan Centromeric Chromosome Paint was employed to recognize centromere positive (CEN+) and negative (CEN-) micronuclei. A total of 1000 binucleate cells (BNC) per participant were analysed after cytochalasin B treatment. All BNC with MN (centromere positive or negative) were recorded by the ISIS software (MetaSystems). No differences were found between formerly exposed workers and the controlgroup, neither in the total frequency of cells with MN per 1000 BNC (mean levels +- standard deviation were 9.1+-3.1 and 9.8+-2.5, resp.), nor in the percentage of CEN- among all MN was nearly equal for both groups (50+-18 and 49+-17, res
Název v anglickém jazyce
Analysis of Genetic Damage in Lymphocytes of Former Uranium Processing Workers
Popis výsledku anglicky
The frequency of cells containing micronuclei (MN) and the presence of centromeres in these MN were analysed in lymphocytes of 98 men from Southern Bohemia. 46 of them had worked at the uranium processing plant, "MAPE Mydlovary", which has been defunct since 1991; 52 men were controls from the same area. Fluorescence in situ hybridization (FISH) method using Human Pan Centromeric Chromosome Paint was employed to recognize centromere positive (CEN+) and negative (CEN-) micronuclei. A total of 1000 binucleate cells (BNC) per participant were analysed after cytochalasin B treatment. All BNC with MN (centromere positive or negative) were recorded by the ISIS software (MetaSystems). No differences were found between formerly exposed workers and the controlgroup, neither in the total frequency of cells with MN per 1000 BNC (mean levels +- standard deviation were 9.1+-3.1 and 9.8+-2.5, resp.), nor in the percentage of CEN- among all MN was nearly equal for both groups (50+-18 and 49+-17, res
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
DN - Vliv životního prostředí na zdraví
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Cytogenetic and Genome Research
ISSN
1424-8581
e-ISSN
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Svazek periodika
147
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
7
Strana od-do
17-23
Kód UT WoS článku
000370491200003
EID výsledku v databázi Scopus
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