Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F10%3A00012481" target="_blank" >RIV/60076658:12220/10:00012481 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography

Popis výsledku v původním jazyce

The simple and reliable method for determination of quercetin glycosides and free quercetin in buckwheat flower, leaves, stems and achenes was developed. The method consists of flavonoid extraction from freeze-dried homogenous material in 50% (v/v) methanol solution and in presence of an antioxidant, cleaning of extract and analyte isolation using solid phase extraction. Analytical step uses capillary micellar electrokinetic chromatography. The working ranges, limit of detection and quantification, recovery, precision and measurement uncertainty were calculated. The method is suitable for samples from buckwheat. The highest content of rutin was found in flowers of both kinds of buckwheat (99 400 mg?kg-1 in F. esculentum, 108 000 99 400 mg?kg-1 in F. tataricum). The free quercetin occurs in flowers and achenes of F. esculentum, while flowers and achenes of F. tataricum contained quercitrin.

Název v anglickém jazyce

Determination of quercetin glycosides and free quercetin in buckwheat by capillary micellar electrokinetic chromatography

Popis výsledku anglicky

The simple and reliable method for determination of quercetin glycosides and free quercetin in buckwheat flower, leaves, stems and achenes was developed. The method consists of flavonoid extraction from freeze-dried homogenous material in 50% (v/v) methanol solution and in presence of an antioxidant, cleaning of extract and analyte isolation using solid phase extraction. Analytical step uses capillary micellar electrokinetic chromatography. The working ranges, limit of detection and quantification, recovery, precision and measurement uncertainty were calculated. The method is suitable for samples from buckwheat. The highest content of rutin was found in flowers of both kinds of buckwheat (99 400 mg?kg-1 in F. esculentum, 108 000 99 400 mg?kg-1 in F. tataricum). The free quercetin occurs in flowers and achenes of F. esculentum, while flowers and achenes of F. tataricum contained quercitrin.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000278829700014

EID výsledku v databázi Scopus

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