Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F10%3A00012493" target="_blank" >RIV/60076658:12220/10:00012493 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress

Popis výsledku v původním jazyce

The durum wheat cultivars ?Cappelli? and ?Ofanto? are characterized by a clear differences in response to heat and drought stress. Wheat Affymetrix microarray was used in the two cultivars subjected to water stress at booting stage. Differential expressed genes with different stress expression profiles were found in Cappelli vs. Ofanto. According to the fold change threshold of the Affymetrix dataset and the annotated activity among these genes drought response gene markers were selected and some of them have been validated by qRT-PCR for their stabile behaving during plant development in an experiment made with two cultivars under drought stress at tillering developmental stages. These results will open the possibility to identify expression QTLs controlling the response of durum wheat to abiotic stress making possible the understanding of the genetic bases of stress dependent gene expression.

Název v anglickém jazyce

Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress

Popis výsledku anglicky

The durum wheat cultivars ?Cappelli? and ?Ofanto? are characterized by a clear differences in response to heat and drought stress. Wheat Affymetrix microarray was used in the two cultivars subjected to water stress at booting stage. Differential expressed genes with different stress expression profiles were found in Cappelli vs. Ofanto. According to the fold change threshold of the Affymetrix dataset and the annotated activity among these genes drought response gene markers were selected and some of them have been validated by qRT-PCR for their stabile behaving during plant development in an experiment made with two cultivars under drought stress at tillering developmental stages. These results will open the possibility to identify expression QTLs controlling the response of durum wheat to abiotic stress making possible the understanding of the genetic bases of stress dependent gene expression.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GD521%2F08%2FH042" target="_blank" >GD521/08/H042: Molekulární biologie, ekologie a taxonomie</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Úroda : časopis pro rostlinnou produkci

ISSN

0139-6013

e-ISSN

—

Svazek periodika

58

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—