THE EXTRACTION OF DNA FROM COLONIES OF PECTINATELLA MAGNIFICA (LEIDY 1851)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F17%3A43896699" target="_blank" >RIV/60076658:12220/17:43896699 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.5593/sgem2017/52/S20.126" target="_blank" >http://dx.doi.org/10.5593/sgem2017/52/S20.126</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5593/sgem2017/52/S20.126" target="_blank" >10.5593/sgem2017/52/S20.126</a>

Jazyk výsledku

angličtina

Název v původním jazyce

THE EXTRACTION OF DNA FROM COLONIES OF PECTINATELLA MAGNIFICA (LEIDY 1851)

Popis výsledku v původním jazyce

Pectinatella magnifica is freshwater organism, which round-shaped colonies are most often found attached to the different submerged parts of coastal plants and can reach the weight even several kilograms. Usually it can be found in oligotrophic to mesotrophic waters, during the season when water temperature reaches 20°C and more [4]. Although Pectinatella magnifica originally comes from North America, it has recently been known as an invasive species in Europe and part of Asia. The first recorded occurrence in Europe was in 1983 near Hamburg [2] in the Czech Republic it was recorded for the first time in rivers Vltava and Labe in the first half of the 20th century [9]. The most common way of its propagation is through internal buds, called statoblasts, which are fitted with hooks. These anchor-shaped hooks make statoblasts able to catch to any surface, and allow easy spread over long distances and different terrain. The aim of this study was to compare different methods of isolation of DNA from colonies of Pectinatella magnifica. Three main different techniques of DNA extraction from zooids and statoblasts were tested, namely two kit-based methods: DNeasy Plant Mini isolation kit (QIAGEN), Nucleo Spin isolation kit (Marcherey-Nagel) and one isolation method based on cetyltrimethylammonium bromide (CTAB-PVP). There were also tested differences between statoblast and zooid DNA and between natural stored and ethanol fixed samples. Material for analysis was collected at the Protected Landscape Area (PLA) and a Biosphere Reserve (BR) of Třeboňsko

Název v anglickém jazyce

THE EXTRACTION OF DNA FROM COLONIES OF PECTINATELLA MAGNIFICA (LEIDY 1851)

Popis výsledku anglicky

Pectinatella magnifica is freshwater organism, which round-shaped colonies are most often found attached to the different submerged parts of coastal plants and can reach the weight even several kilograms. Usually it can be found in oligotrophic to mesotrophic waters, during the season when water temperature reaches 20°C and more [4]. Although Pectinatella magnifica originally comes from North America, it has recently been known as an invasive species in Europe and part of Asia. The first recorded occurrence in Europe was in 1983 near Hamburg [2] in the Czech Republic it was recorded for the first time in rivers Vltava and Labe in the first half of the 20th century [9]. The most common way of its propagation is through internal buds, called statoblasts, which are fitted with hooks. These anchor-shaped hooks make statoblasts able to catch to any surface, and allow easy spread over long distances and different terrain. The aim of this study was to compare different methods of isolation of DNA from colonies of Pectinatella magnifica. Three main different techniques of DNA extraction from zooids and statoblasts were tested, namely two kit-based methods: DNeasy Plant Mini isolation kit (QIAGEN), Nucleo Spin isolation kit (Marcherey-Nagel) and one isolation method based on cetyltrimethylammonium bromide (CTAB-PVP). There were also tested differences between statoblast and zooid DNA and between natural stored and ethanol fixed samples. Material for analysis was collected at the Protected Landscape Area (PLA) and a Biosphere Reserve (BR) of Třeboňsko

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Int. Multidisciplinary Sci.Geoconf. Surveying Geology Mining Ecology Manage.,SGEM

ISBN

978-619-7408-09-6

ISSN

1314-2704

e-ISSN

neuvedeno

Počet stran výsledku

8

Strana od-do

989-996

Název nakladatele

International Multidisciplinary Scientific Geoconference

Místo vydání

Albena

Místo konání akce

Albena

Datum konání akce

29. 6. 2017

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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