INFLUENCE OF HUSK ON GRAIN CONTAMINATION BY Fusarium spp. AND Alternaria spp. IN HULLED SPELT (Triticum spelta L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F18%3A43898622" target="_blank" >RIV/60076658:12220/18:43898622 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/18:77519 RIV/00027006:_____/18:00004565

Výsledek na webu

<a href="http://dx.doi.org/10.30638/eemj.2018.089" target="_blank" >http://dx.doi.org/10.30638/eemj.2018.089</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.30638/eemj.2018.089" target="_blank" >10.30638/eemj.2018.089</a>

Jazyk výsledku

angličtina

Název v původním jazyce

INFLUENCE OF HUSK ON GRAIN CONTAMINATION BY Fusarium spp. AND Alternaria spp. IN HULLED SPELT (Triticum spelta L.)

Popis výsledku v původním jazyce

Fusarium Head Blight is caused by several Fusarium species. Infections can result in mycotoxin contamination on cereals and associated foods. Harvested products are contaminated due to its secondary metabolites. The aim was to analyse the occurrence of spike Fusarium and Alternaria spp. in hulled Triticum spelta L. wheat species via polymerase chain reaction (PCR) method and the deoxynivalenol (DON) content analysis. Three varieties of spelt were used (Ceralio and Rubiota – winter and one spring form variety from genetics resources). Grains were sown in a randomized complete block design on organic certified experimental parcels during the years of 2011 and 2013. During the vegetation period plants were artificially inoculated with Fusarium spp. The occurrence of spike Fusarium and Alternaria spp. was assessed by the PCR method - DNA extracting and determination of Fusarium species and Alternaria spp. by the DNA markers and PCR method. DON contenwas analysed by ROSA®-DON Quantitative test. Strong infestation of grains with Fusarium spp. led to low contamination of grains with Alternaria spp. The technological operation of grain dehulling was performed and it was highly efficient there. The grain contamination by Fusarium spp. and Alternaria spp. decreased. Hulls protect grains to a certain point because of protection against Fusarium spp. and Alternaria spp. occurrence which produce harmful secondary metabolites. On the other hand the protection of grain by hulls only partly works. It is also important to pay attention to chemism of secondary metabolites in grain.

Název v anglickém jazyce

INFLUENCE OF HUSK ON GRAIN CONTAMINATION BY Fusarium spp. AND Alternaria spp. IN HULLED SPELT (Triticum spelta L.)

Popis výsledku anglicky

Fusarium Head Blight is caused by several Fusarium species. Infections can result in mycotoxin contamination on cereals and associated foods. Harvested products are contaminated due to its secondary metabolites. The aim was to analyse the occurrence of spike Fusarium and Alternaria spp. in hulled Triticum spelta L. wheat species via polymerase chain reaction (PCR) method and the deoxynivalenol (DON) content analysis. Three varieties of spelt were used (Ceralio and Rubiota – winter and one spring form variety from genetics resources). Grains were sown in a randomized complete block design on organic certified experimental parcels during the years of 2011 and 2013. During the vegetation period plants were artificially inoculated with Fusarium spp. The occurrence of spike Fusarium and Alternaria spp. was assessed by the PCR method - DNA extracting and determination of Fusarium species and Alternaria spp. by the DNA markers and PCR method. DON contenwas analysed by ROSA®-DON Quantitative test. Strong infestation of grains with Fusarium spp. led to low contamination of grains with Alternaria spp. The technological operation of grain dehulling was performed and it was highly efficient there. The grain contamination by Fusarium spp. and Alternaria spp. decreased. Hulls protect grains to a certain point because of protection against Fusarium spp. and Alternaria spp. occurrence which produce harmful secondary metabolites. On the other hand the protection of grain by hulls only partly works. It is also important to pay attention to chemism of secondary metabolites in grain.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40101 - Agriculture

Projekt

<a href="/cs/project/QJ1310072" target="_blank" >QJ1310072: Využití systému participatory breeding ve výzkumu a šlechtění odrůd pšenice vhodných pro ekologické pěstování</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Engineering and Management Journal

ISSN

1582-9596

e-ISSN

—

Svazek periodika

17

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

RO - Rumunsko

Počet stran výsledku

11

Strana od-do

885-895

Kód UT WoS článku

000431134900014

EID výsledku v databázi Scopus

2-s2.0-85047155750