Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F19%3A43899021" target="_blank" >RIV/60076658:12220/19:43899021 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/19:00519676 RIV/60076658:12310/19:43899021

Výsledek na webu

<a href="https://www.cambridge.org/core/journals/parasitology/article/diversity-of-cryptosporidium-in-common-voles-and-description-of-cryptosporidium-alticolis-sp-n-and-cryptosporidium-microti-sp-n-apicomplexa-cryptosporidiidae/9667CA24CCAE50F331A284F0C58F861F" target="_blank" >https://www.cambridge.org/core/journals/parasitology/article/diversity-of-cryptosporidium-in-common-voles-and-description-of-cryptosporidium-alticolis-sp-n-and-cryptosporidium-microti-sp-n-apicomplexa-cryptosporidiidae/9667CA24CCAE50F331A284F0C58F861F</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0031182018001142" target="_blank" >10.1017/S0031182018001142</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

Popis výsledku v původním jazyce

Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 x 4.9 mu m), whereas C. microti infects the large intestine and has smaller oocysts (4.3 x 4.1 mu m). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.

Název v anglickém jazyce

Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

Popis výsledku anglicky

Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3-5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11-14 vs 13-16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 x 4.9 mu m), whereas C. microti infects the large intestine and has smaller oocysts (4.3 x 4.1 mu m). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology

ISSN

0031-1820

e-ISSN

—

Svazek periodika

146

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

220-233

Kód UT WoS článku

000458677800010

EID výsledku v databázi Scopus

2-s2.0-85049975640