Photobiochemical changes in Chlorellag120 culture during trophic conversion (metabolic pathway shift) from heterotrophic to phototrophic growth regime

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F20%3A43901429" target="_blank" >RIV/60076658:12220/20:43901429 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/20:00533409 RIV/60076658:12310/20:43901429

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10811-020-02137-w" target="_blank" >https://link.springer.com/article/10.1007/s10811-020-02137-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10811-020-02137-w" target="_blank" >10.1007/s10811-020-02137-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Photobiochemical changes in Chlorellag120 culture during trophic conversion (metabolic pathway shift) from heterotrophic to phototrophic growth regime

Popis výsledku v původním jazyce

Physiological and photobiochemical changes and growth in the heterotrophic strainChlorella vulgarisg120 were studied during trophic conversion from heterotrophic to phototrophic growth regime. After the exposure of theChlorellag120 culture to light, it revealed a significant activity of the electron transport (450-700 mu mol e(-) m(-2) s(-1)as measured by chlorophyll fluorescence) and high PSII photochemical yieldF(v)/F(m)between 0.7 and 0.8. Fast fluorescence induction kinetics showed that PSII electron acceptors in the plastoquinone pool remained partly oxidized, indicating no downregulation of PSII electron transport. The data further revealed that high photobiochemical activity is lost in futile (protective) processes of non-photochemical quenching and respiration which indicate that surplus energy is dissipated in these processes. Pigment analysis showed low chlorophyll content - 0.35-1.15% as compared with exclusively phototrophic strainChlorella vulgarisR-117. Nevertheless, the carotenoid content in g120 was relatively high - 0.20-0.33% of dry weight which resulted in a considerably high ratio of carotenoid/chlorophyll - 0.31-0.44. This strain probably does not possess the fully competent photosynthetic apparatus and can only partially adapt to phototrophy. We show that the heterotrophically grown g120 strain can undergo metabolic shift from heterotrophic to phototrophic growth regime. It might be an interesting strain from biotechnological point of view as a source of carotenoids, especially lutein.

Název v anglickém jazyce

Photobiochemical changes in Chlorellag120 culture during trophic conversion (metabolic pathway shift) from heterotrophic to phototrophic growth regime

Popis výsledku anglicky

Physiological and photobiochemical changes and growth in the heterotrophic strainChlorella vulgarisg120 were studied during trophic conversion from heterotrophic to phototrophic growth regime. After the exposure of theChlorellag120 culture to light, it revealed a significant activity of the electron transport (450-700 mu mol e(-) m(-2) s(-1)as measured by chlorophyll fluorescence) and high PSII photochemical yieldF(v)/F(m)between 0.7 and 0.8. Fast fluorescence induction kinetics showed that PSII electron acceptors in the plastoquinone pool remained partly oxidized, indicating no downregulation of PSII electron transport. The data further revealed that high photobiochemical activity is lost in futile (protective) processes of non-photochemical quenching and respiration which indicate that surplus energy is dissipated in these processes. Pigment analysis showed low chlorophyll content - 0.35-1.15% as compared with exclusively phototrophic strainChlorella vulgarisR-117. Nevertheless, the carotenoid content in g120 was relatively high - 0.20-0.33% of dry weight which resulted in a considerably high ratio of carotenoid/chlorophyll - 0.31-0.44. This strain probably does not possess the fully competent photosynthetic apparatus and can only partially adapt to phototrophy. We show that the heterotrophically grown g120 strain can undergo metabolic shift from heterotrophic to phototrophic growth regime. It might be an interesting strain from biotechnological point of view as a source of carotenoids, especially lutein.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

<a href="/cs/project/LO1416" target="_blank" >LO1416: Algatech plus</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Phycology

ISSN

0921-8971

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

2807-2818

Kód UT WoS článku

000539524100001

EID výsledku v databázi Scopus

2-s2.0-85086317714