Real-time loop-mediated isothermal amplification assay for rapid detection of Fusarium circinatum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F20%3A43901649" target="_blank" >RIV/60076658:12220/20:43901649 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.future-science.com/doi/full/10.2144/btn-2019-0168" target="_blank" >https://www.future-science.com/doi/full/10.2144/btn-2019-0168</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2144/btn-2019-0168" target="_blank" >10.2144/btn-2019-0168</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Real-time loop-mediated isothermal amplification assay for rapid detection of Fusarium circinatum

Popis výsledku v původním jazyce

Fusarium circinatum is the causal agent of pitch canker, a lethal disease of pine and other conifers. Since F. circinatum is a quarantine organism, its timely detection could efficiently prevent its introduction into new areas or facilitate spread management in already infected sites. In this study, we developed a sequence-specific probe loop-mediated isothermal amplification (LAMP) assay for F. circinatum using a field-deployable portable instrument. The assay was able to recognize the pathogen in host tissues in just 30 min, and the sensitivity of the assay made it possible to detect even small amounts of F. circinatum DNA (as low as 0.5 pg/mu l). The high efficiency of this method suggests its use as a standard diagnostic tool during phytosanitary controls.

Název v anglickém jazyce

Real-time loop-mediated isothermal amplification assay for rapid detection of Fusarium circinatum

Popis výsledku anglicky

Fusarium circinatum is the causal agent of pitch canker, a lethal disease of pine and other conifers. Since F. circinatum is a quarantine organism, its timely detection could efficiently prevent its introduction into new areas or facilitate spread management in already infected sites. In this study, we developed a sequence-specific probe loop-mediated isothermal amplification (LAMP) assay for F. circinatum using a field-deployable portable instrument. The assay was able to recognize the pathogen in host tissues in just 30 min, and the sensitivity of the assay made it possible to detect even small amounts of F. circinatum DNA (as low as 0.5 pg/mu l). The high efficiency of this method suggests its use as a standard diagnostic tool during phytosanitary controls.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BioTechniques

ISSN

0736-6205

e-ISSN

—

Svazek periodika

69

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

11-17

Kód UT WoS článku

000588650200004

EID výsledku v databázi Scopus

—