Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the FirstWeek of Germination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F21%3A43902921" target="_blank" >RIV/60076658:12220/21:43902921 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61988987:17310/21:A2202ATN RIV/26791251:_____/21:N0000029 RIV/00216224:14740/21:00124441

Výsledek na webu

<a href="https://www.mdpi.com/2223-7747/10/9/1878" target="_blank" >https://www.mdpi.com/2223-7747/10/9/1878</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/plants10091878" target="_blank" >10.3390/plants10091878</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the FirstWeek of Germination

Popis výsledku v původním jazyce

Water deficiency is one of the most significant abiotic stresses that negatively affects growth and reduces crop yields worldwide. Most research is focused on model plants and/or crops which are most agriculturally important. In this research, drought stress was applied to two drought stress contrasting varieties of Papaver somniferum (the opium poppy), a non-model plant species, during the first week of its germination, which differ in responses to drought stress. After sowing, the poppy seedlings were immediately subjected to drought stress for 7 days. We conducted a large-scale transcriptomic and proteomic analysis for drought stress response. At first, we found that the transcriptomic and proteomic profiles significantly differ. However, the most significant findings are the identification of key genes and proteins with significantly different expressions relating to drought stress, e.g., the heat-shock protein family, dehydration responsive elementbinding transcription factors, ubiquitin E3 ligase, and others. In addition, metabolic pathway analysis showed that these genes and proteins were part of several biosynthetic pathways most significantly related to photosynthetic processes, and oxidative stress responses. A future study will focus on a detailed analysis of key genes and the development of selection markers for the determination of drought-resistant varieties and the breeding of new resistant lineages.

Název v anglickém jazyce

Transcriptomic and Proteomic Analysis of Drought Stress Response in Opium Poppy Plants during the FirstWeek of Germination

Popis výsledku anglicky

Water deficiency is one of the most significant abiotic stresses that negatively affects growth and reduces crop yields worldwide. Most research is focused on model plants and/or crops which are most agriculturally important. In this research, drought stress was applied to two drought stress contrasting varieties of Papaver somniferum (the opium poppy), a non-model plant species, during the first week of its germination, which differ in responses to drought stress. After sowing, the poppy seedlings were immediately subjected to drought stress for 7 days. We conducted a large-scale transcriptomic and proteomic analysis for drought stress response. At first, we found that the transcriptomic and proteomic profiles significantly differ. However, the most significant findings are the identification of key genes and proteins with significantly different expressions relating to drought stress, e.g., the heat-shock protein family, dehydration responsive elementbinding transcription factors, ubiquitin E3 ligase, and others. In addition, metabolic pathway analysis showed that these genes and proteins were part of several biosynthetic pathways most significantly related to photosynthetic processes, and oxidative stress responses. A future study will focus on a detailed analysis of key genes and the development of selection markers for the determination of drought-resistant varieties and the breeding of new resistant lineages.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QK1810391" target="_blank" >QK1810391: Využití technik genomiky a transkriptomiky k tvorbě genových zdrojů a výchozích materiálů máku se specifickými vlastnostmi</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plants-Basel

ISSN

2223-7747

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

1878

Kód UT WoS článku

000701583900001

EID výsledku v databázi Scopus

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