Development of RT-PCR for rapid detection of ssRNA ambi-like mycovirus in a root rot fungi (Armillaria spp.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F22%3A43905896" target="_blank" >RIV/60076658:12220/22:43905896 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7828&category_id=184&option=com_virtuemart" target="_blank" >http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7828&category_id=184&option=com_virtuemart</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/av_2022_308" target="_blank" >10.4149/av_2022_308</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of RT-PCR for rapid detection of ssRNA ambi-like mycovirus in a root rot fungi (Armillaria spp.)

Popis výsledku v původním jazyce

Plant pathogenic fungi cause severe losses and damages to crops worldwide. Fungal antagonists including viruses can play a significant role in controlling of these phytopathogens and reduce disease risk in plants. Understanding the mode of action of mycoviruses is essential to achieve optimum host control. Although the number of described mycoviruses is increasing due to the ability to detect different mycoviruses using transcriptome approaches, there is a need to identify and isolate new viruses from phytopathogenic fungi that can introduce new possibilities to control serious plants pests. The rapid, reliable and cost-effective screening of fungi is required when searching for new potentially effective biological control products. Here, we evaluated the efficacy of the reverse transcription PCR (RT-PCR) for fast detection using samples from Armillaria sp. mycovirus survey. After testing 12 strains of Armillaria spp., 2 were found to be positive for ambisense viruses by RT-PCR. Ambisense ssRNA viruses was detectable in all symptomless mycelia, including strains maintained in fungal culture collection for decades.

Název v anglickém jazyce

Development of RT-PCR for rapid detection of ssRNA ambi-like mycovirus in a root rot fungi (Armillaria spp.)

Popis výsledku anglicky

Plant pathogenic fungi cause severe losses and damages to crops worldwide. Fungal antagonists including viruses can play a significant role in controlling of these phytopathogens and reduce disease risk in plants. Understanding the mode of action of mycoviruses is essential to achieve optimum host control. Although the number of described mycoviruses is increasing due to the ability to detect different mycoviruses using transcriptome approaches, there is a need to identify and isolate new viruses from phytopathogenic fungi that can introduce new possibilities to control serious plants pests. The rapid, reliable and cost-effective screening of fungi is required when searching for new potentially effective biological control products. Here, we evaluated the efficacy of the reverse transcription PCR (RT-PCR) for fast detection using samples from Armillaria sp. mycovirus survey. After testing 12 strains of Armillaria spp., 2 were found to be positive for ambisense viruses by RT-PCR. Ambisense ssRNA viruses was detectable in all symptomless mycelia, including strains maintained in fungal culture collection for decades.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/QK1920412" target="_blank" >QK1920412: Mykoviry jako součást potenciálních biopreparátů v ochraně smrkových porostů proti václavkám</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Virologica

ISSN

0001-723X

e-ISSN

—

Svazek periodika

66

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

3

Strana od-do

287-289

Kód UT WoS článku

000884779500010

EID výsledku v databázi Scopus

2-s2.0-85138452316