Understanding the molecular mechanisms of interactions between biochar and denitrifiers in N₂O emissions reduction: Pathway to more economical and sustainable fertilizers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F25%3A43908547" target="_blank" >RIV/60076658:12220/25:43908547 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12510/25:43908547

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0167198724004069" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0167198724004069</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.still.2024.106405" target="_blank" >10.1016/j.still.2024.106405</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Understanding the molecular mechanisms of interactions between biochar and denitrifiers in N₂O emissions reduction: Pathway to more economical and sustainable fertilizers

Popis výsledku v původním jazyce

Biochar application to topsoil has been repeatedly and independently reported to reduce N2O emissions, yet the underlying mechanisms remain poorly understood. This study hypothesizes that biochar enhances the stability and catalytic activity of N2O reductase enzymes in denitrifying bacteria, promoting the conversion of N2O to N2 during denitrification. Interactions between biochar and the N2O reductase enzyme (PsN2OR) from the denitrifying bacterium Pseudomonas stutzeri were investigated through molecular dynamics simulations. The obtained results firstly revealed that biochar stabilizes this periplasmic enzyme in the aqueous solution via hydrophobic and hydrophilic interactions. Specifically, π–π stacking and hydrophobic interactions reduce the thermal fluctuations of hydrophobic amino acids, lowering entropy and improving enzymatic efficiency. Additionally, biochar adsorbs N2O molecules, facilitating their delivery to the active site of the enzyme and enhancing the reaction rate. Deeper understandings of molecular interactions open new pathways in developing biochar-based fertilizers with slower, more economically and more environmentally favorable release of nutrients. This new type of fertilizers creates new opportunities for the biochar market, positioning it as a valuable tool for carbon sequestration and the mitigation of N₂O emissions.

Název v anglickém jazyce

Understanding the molecular mechanisms of interactions between biochar and denitrifiers in N₂O emissions reduction: Pathway to more economical and sustainable fertilizers

Popis výsledku anglicky

Biochar application to topsoil has been repeatedly and independently reported to reduce N2O emissions, yet the underlying mechanisms remain poorly understood. This study hypothesizes that biochar enhances the stability and catalytic activity of N2O reductase enzymes in denitrifying bacteria, promoting the conversion of N2O to N2 during denitrification. Interactions between biochar and the N2O reductase enzyme (PsN2OR) from the denitrifying bacterium Pseudomonas stutzeri were investigated through molecular dynamics simulations. The obtained results firstly revealed that biochar stabilizes this periplasmic enzyme in the aqueous solution via hydrophobic and hydrophilic interactions. Specifically, π–π stacking and hydrophobic interactions reduce the thermal fluctuations of hydrophobic amino acids, lowering entropy and improving enzymatic efficiency. Additionally, biochar adsorbs N2O molecules, facilitating their delivery to the active site of the enzyme and enhancing the reaction rate. Deeper understandings of molecular interactions open new pathways in developing biochar-based fertilizers with slower, more economically and more environmentally favorable release of nutrients. This new type of fertilizers creates new opportunities for the biochar market, positioning it as a valuable tool for carbon sequestration and the mitigation of N₂O emissions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

50202 - Applied Economics, Econometrics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Soil and Tillage Research

ISSN

0167-1987

e-ISSN

1879-3444

Svazek periodika

248

Číslo periodika v rámci svazku

2025

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

1-6

Kód UT WoS článku

001382308800001

EID výsledku v databázi Scopus

2-s2.0-85211069101