Development of an algae extract-based culture medium for Paenibacillus larvae without animal-derived components

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F25%3A43910226" target="_blank" >RIV/60076658:12220/25:43910226 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2215016125004820?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2215016125004820?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mex.2025.103638" target="_blank" >10.1016/j.mex.2025.103638</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of an algae extract-based culture medium for Paenibacillus larvae without animal-derived components

Popis výsledku v původním jazyce

The cultivation of Paenibacillus larvae, the etiological agent of American foulbrood, traditionally relies on media containing animal-derived nutrients. In response to ethical and practical concerns associated with such components, we developed and validated a new culture medium based on an algae extract. The formulation includes Chlorella as the principal nutrient source and is supplemented with uric acid and L-tyrosine to support spore germination under conditions resembling the honeybee larval gut. The method includes optimized procedures for dissolving sparingly soluble components and minimizing nutrient degradation. Performance of the new medium was comparable to that of MYPGP and other conventional media in supporting spore germination and vegetative growth of P. larvae.• Eliminates the need for animal-derived components through the use of microalgal nutrients• Supports robust germination and colony development of P. Larvae• Provides an ethical and cost-efficient medium suitable for diagnostic and research applications

Název v anglickém jazyce

Development of an algae extract-based culture medium for Paenibacillus larvae without animal-derived components

Popis výsledku anglicky

The cultivation of Paenibacillus larvae, the etiological agent of American foulbrood, traditionally relies on media containing animal-derived nutrients. In response to ethical and practical concerns associated with such components, we developed and validated a new culture medium based on an algae extract. The formulation includes Chlorella as the principal nutrient source and is supplemented with uric acid and L-tyrosine to support spore germination under conditions resembling the honeybee larval gut. The method includes optimized procedures for dissolving sparingly soluble components and minimizing nutrient degradation. Performance of the new medium was comparable to that of MYPGP and other conventional media in supporting spore germination and vegetative growth of P. larvae.• Eliminates the need for animal-derived components through the use of microalgal nutrients• Supports robust germination and colony development of P. Larvae• Provides an ethical and cost-efficient medium suitable for diagnostic and research applications

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MethodsX

ISSN

—

e-ISSN

2215-0161

Svazek periodika

15

Číslo periodika v rámci svazku

103638

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

1-5

Kód UT WoS článku

001584893800002

EID výsledku v databázi Scopus

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