Effect of pH and dissolved organic matter on the abundance of nirK and nirS denitrifiers in spruce forest soil

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F10%3A00011888" target="_blank" >RIV/60076658:12310/10:00011888 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of pH and dissolved organic matter on the abundance of nirK and nirS denitrifiers in spruce forest soil

Popis výsledku v původním jazyce

Acid N depositions in the Bohemian Forest during the second half of the last century caused enormous soil acidification which led to the leaching of essential nutrients including nitrates. We investigated the effect of dissolved organic matter (DOM) andpH on the abundance of 16S RDNA, nirK and nirS gene copies in four spruce forest sites. Soil samples for molecular based quantification (qPCR) were taken from the organic litter and humus layers. The amounts of dissolved organic carbon (DOC) and dissolved nitrogen (DN) were much lower in highly acidified soils. We found a strong correlation between nirK denitrifiers and the amount of available P (r = 0.83, p < 0.001), which suggested a higher nutrient sensitivity of this group of denitrifying bacteria.Additionally, we found that correlations between the amount of nirK denitrifiers and DOC and pH are exponentional showing two important threshold values, being 4.8 mol kg(-1) and 5, respectively. The amount of nirK denitrifiers rapidly de

Název v anglickém jazyce

Effect of pH and dissolved organic matter on the abundance of nirK and nirS denitrifiers in spruce forest soil

Popis výsledku anglicky

Acid N depositions in the Bohemian Forest during the second half of the last century caused enormous soil acidification which led to the leaching of essential nutrients including nitrates. We investigated the effect of dissolved organic matter (DOM) andpH on the abundance of 16S RDNA, nirK and nirS gene copies in four spruce forest sites. Soil samples for molecular based quantification (qPCR) were taken from the organic litter and humus layers. The amounts of dissolved organic carbon (DOC) and dissolved nitrogen (DN) were much lower in highly acidified soils. We found a strong correlation between nirK denitrifiers and the amount of available P (r = 0.83, p < 0.001), which suggested a higher nutrient sensitivity of this group of denitrifying bacteria.Additionally, we found that correlations between the amount of nirK denitrifiers and DOC and pH are exponentional showing two important threshold values, being 4.8 mol kg(-1) and 5, respectively. The amount of nirK denitrifiers rapidly de

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biogeochemistry

ISSN

0168-2563

e-ISSN

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Svazek periodika

101

Číslo periodika v rámci svazku

1-3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000285096700010

EID výsledku v databázi Scopus

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