Image cytometric measurements of diploid, triploid and tetraploid fish erythrocytes in blood smears reflect the true dimensions of live cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F11%3A43873469" target="_blank" >RIV/60076658:12310/11:43873469 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12520/11:43873469

Výsledek na webu

<a href="http://dx.doi.org/10.1042/CBI20100198" target="_blank" >http://dx.doi.org/10.1042/CBI20100198</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1042/CBI20100198" target="_blank" >10.1042/CBI20100198</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Image cytometric measurements of diploid, triploid and tetraploid fish erythrocytes in blood smears reflect the true dimensions of live cells

Popis výsledku v původním jazyce

Comparative image cytometry of erythrocytes of diploid (2n) and triploid (3n) tench and evolutionary tetraploid (4n) sterlet was performed on whole live unstained cells, live cells with stained nuclei and on stained fixed cells and their nuclei to test if erythrocyte measurements made from blood smears reflect the true dimensions of live cells. Nuclear area and perimeter were the best ploidy level predictors distinguishing accurately among live and fixed 2n, 3n and 4n cells, without significant differences between live and fixed cells within a ploidy level. Redundancy analysis revealed insignificant marginal effect of fixation (explained 2.3 % of variation, F=0.804), the effect of ploidy level was highly significant (explained 50.6 % of variation, F=34.874). Erythrocyte measurements of 2n, 3n and 4n fish erythrocytes and their nuclei from blood smears reflected true dimensions of live cells, and the fixation procedure did not substantially affect their predictive value for ploidy level

Název v anglickém jazyce

Image cytometric measurements of diploid, triploid and tetraploid fish erythrocytes in blood smears reflect the true dimensions of live cells

Popis výsledku anglicky

Comparative image cytometry of erythrocytes of diploid (2n) and triploid (3n) tench and evolutionary tetraploid (4n) sterlet was performed on whole live unstained cells, live cells with stained nuclei and on stained fixed cells and their nuclei to test if erythrocyte measurements made from blood smears reflect the true dimensions of live cells. Nuclear area and perimeter were the best ploidy level predictors distinguishing accurately among live and fixed 2n, 3n and 4n cells, without significant differences between live and fixed cells within a ploidy level. Redundancy analysis revealed insignificant marginal effect of fixation (explained 2.3 % of variation, F=0.804), the effect of ploidy level was highly significant (explained 50.6 % of variation, F=34.874). Erythrocyte measurements of 2n, 3n and 4n fish erythrocytes and their nuclei from blood smears reflected true dimensions of live cells, and the fixation procedure did not substantially affect their predictive value for ploidy level

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cell Biology International

ISSN

1065-6995

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

67-71

Kód UT WoS článku

000290283100009

EID výsledku v databázi Scopus

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