Different temperature sensitivity and kinetics of soil enzymes indicate seasonal shifts in C, N and P nutrient stoichiometry in acid forest soil

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F14%3A43887145" target="_blank" >RIV/60076658:12310/14:43887145 - isvavai.cz</a>

Výsledek na webu

<a href="http://download.springer.com/static/pdf/218/art%253A10.1007%252Fs10533-013-9898-1.pdf?auth66=1422182864_84084019bd7a1f07c698f5fcb16d85ad&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/218/art%253A10.1007%252Fs10533-013-9898-1.pdf?auth66=1422182864_84084019bd7a1f07c698f5fcb16d85ad&ext=.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10533-013-9898-1" target="_blank" >10.1007/s10533-013-9898-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Different temperature sensitivity and kinetics of soil enzymes indicate seasonal shifts in C, N and P nutrient stoichiometry in acid forest soil

Popis výsledku v původním jazyce

Acid forest soils in the Bohemian Forest in Central Europe are biogeochemically imbalanced in organic C, N and P processing. We hypothesized that these imbalances can be due to different temperature sensitivities of soil enzyme activities and their affinities to substrate in litter and organic soil horizons. We measured potential activities of five main soil enzymes (beta-glucosidase, cellobiohydrolase, Leu-aminopeptidase, Ala-aminopeptidase, and phosphatase) responsible for organic carbon, nitrogen andphosphorus acquisition. We also modeled potential in situ enzyme activities and nutrient release based on continuous in situ temperature measurements. We determined basic kinetic parameters (K-m, V-max), enzyme efficiencies (k(cat)) and temperature sensitivities (E-a and Q(10)) according to Michaelis-Menten kinetic and modified Arrhenius models. Our results showed significant differences in substrate affinities between the litter and organic soil horizons. Higher aminopeptidase affinity

Název v anglickém jazyce

Different temperature sensitivity and kinetics of soil enzymes indicate seasonal shifts in C, N and P nutrient stoichiometry in acid forest soil

Popis výsledku anglicky

Acid forest soils in the Bohemian Forest in Central Europe are biogeochemically imbalanced in organic C, N and P processing. We hypothesized that these imbalances can be due to different temperature sensitivities of soil enzyme activities and their affinities to substrate in litter and organic soil horizons. We measured potential activities of five main soil enzymes (beta-glucosidase, cellobiohydrolase, Leu-aminopeptidase, Ala-aminopeptidase, and phosphatase) responsible for organic carbon, nitrogen andphosphorus acquisition. We also modeled potential in situ enzyme activities and nutrient release based on continuous in situ temperature measurements. We determined basic kinetic parameters (K-m, V-max), enzyme efficiencies (k(cat)) and temperature sensitivities (E-a and Q(10)) according to Michaelis-Menten kinetic and modified Arrhenius models. Our results showed significant differences in substrate affinities between the litter and organic soil horizons. Higher aminopeptidase affinity

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA526%2F08%2F0751" target="_blank" >GA526/08/0751: Půdní procesy a mikrobní společenstva se vztahem k cyklům C a N v průběhu regenerace horského smrkového lesa po kůrovcové kalamitě</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biogeochemistry

ISSN

0168-2563

e-ISSN

—

Svazek periodika

117

Číslo periodika v rámci svazku

2-3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

525-537

Kód UT WoS článku

000333703400019

EID výsledku v databázi Scopus

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