Accurate Determination of the Orientational Distribution of a Fluorescent Molecule in a Phospholipid Membrane

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F14%3A43887349" target="_blank" >RIV/60076658:12310/14:43887349 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/14:00427648 RIV/61388955:_____/14:00427648 RIV/61388963:_____/14:00427648

Výsledek na webu

<a href="http://dx.doi.org/10.1021/jp4067026" target="_blank" >http://dx.doi.org/10.1021/jp4067026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/jp4067026" target="_blank" >10.1021/jp4067026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Accurate Determination of the Orientational Distribution of a Fluorescent Molecule in a Phospholipid Membrane

Popis výsledku v původním jazyce

Orientation of lipophilic dye molecules within a biological membrane can report on the molecular environment, i.e., the physical and chemical properties of the surrounding membrane. This fact, however, remains under-utilized, largely because of our limited quantitative knowledge of molecular orientational distributions and the fact that robust techniques allowing experimental observation of molecular orientations of dyes in biological membranes are only being developed. In order to begin filling this lack of knowledge and to develop appropriate tools, we have investigated the membrane orientational distribution of the 3-hydroxyflavone-based membrane dye F2N12S. Results of our single- and two-photon polarization microscopy observations of linear dichroism of F2N12S-labeled giant unilamellar vesicles are consistent with a Gaussian-like orientational distribution of the transition dipole moment of the dye, with a mean tilt angle of 53.2 +/- 0.1 degrees with respect to the bilayer normal a

Název v anglickém jazyce

Accurate Determination of the Orientational Distribution of a Fluorescent Molecule in a Phospholipid Membrane

Popis výsledku anglicky

Orientation of lipophilic dye molecules within a biological membrane can report on the molecular environment, i.e., the physical and chemical properties of the surrounding membrane. This fact, however, remains under-utilized, largely because of our limited quantitative knowledge of molecular orientational distributions and the fact that robust techniques allowing experimental observation of molecular orientations of dyes in biological membranes are only being developed. In order to begin filling this lack of knowledge and to develop appropriate tools, we have investigated the membrane orientational distribution of the 3-hydroxyflavone-based membrane dye F2N12S. Results of our single- and two-photon polarization microscopy observations of linear dichroism of F2N12S-labeled giant unilamellar vesicles are consistent with a Gaussian-like orientational distribution of the transition dipole moment of the dye, with a mean tilt angle of 53.2 +/- 0.1 degrees with respect to the bilayer normal a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physical Chemistry B

ISSN

1520-6106

e-ISSN

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Svazek periodika

118

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

855-863

Kód UT WoS článku

000330610400001

EID výsledku v databázi Scopus

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