Efficient light-harvesting using non-carbonyl carotenoids: Energy transfer dynamics in the VCP complex from Nannochloropsis oceanica

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F16%3A43890603" target="_blank" >RIV/60076658:12310/16:43890603 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/16:00460502

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S000527281500273X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S000527281500273X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbabio.2015.12.011" target="_blank" >10.1016/j.bbabio.2015.12.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Efficient light-harvesting using non-carbonyl carotenoids: Energy transfer dynamics in the VCP complex from Nannochloropsis oceanica

Popis výsledku v původním jazyce

Violaxanthin-chlorophyll a protein (VCP) from Nannochloropsis oceanica is a Chl a-only member of the LHC family of light-harvesting proteins. VCP binds carotenoids violaxanthin (Vio), vaucheriaxanthin (Vau), and vaucheriaxanthin-ester (Vau-ester). Here we report on energy transfer pathways in the VCP complex. The overall carotenoid-to-Chla energy transfer has efficiency over 90%. Based on their energy transfer properties, the carotenoids in VCP can be divided into two groups; blue carotenoids with the lowest energy absorption band around 480 nm and red carotenoids with absorption extended up to 530 nm. Both carotenoid groups transfer energy efficiently from their S-2 states, reaching efficiencies of similar to 70% (blue) and similar to 60% (red). The S-1 pathway, however, is efficient only for the red carotenoid pool for which two S-1 routes characterized by 0.33 and 2.4 ps time constants were identified. For the blue carotenoids the S-1-mediated pathway is represented only by a minor route likely involving a hot S-1 state. The relaxed S-1 state of blue carotenoids decays to the ground state within 21 ps. Presence of a fraction of non-transferring red carotenoids with the S-1 lifetime of 13 ps indicates some specific carotenoid-protein interaction that must shorten the intrinsic S-1 lifetime of Vio and/or Vau whose S-1 lifetimes in methanol are 26 and 29 ps, respectively. The VCP complex from N. oceanica is the first example of a light-harvesting complex binding only non-carbonyl carotenoids with carotenoid-to-chlorophyll energy transfer efficiency over 90%.

Název v anglickém jazyce

Efficient light-harvesting using non-carbonyl carotenoids: Energy transfer dynamics in the VCP complex from Nannochloropsis oceanica

Popis výsledku anglicky

Violaxanthin-chlorophyll a protein (VCP) from Nannochloropsis oceanica is a Chl a-only member of the LHC family of light-harvesting proteins. VCP binds carotenoids violaxanthin (Vio), vaucheriaxanthin (Vau), and vaucheriaxanthin-ester (Vau-ester). Here we report on energy transfer pathways in the VCP complex. The overall carotenoid-to-Chla energy transfer has efficiency over 90%. Based on their energy transfer properties, the carotenoids in VCP can be divided into two groups; blue carotenoids with the lowest energy absorption band around 480 nm and red carotenoids with absorption extended up to 530 nm. Both carotenoid groups transfer energy efficiently from their S-2 states, reaching efficiencies of similar to 70% (blue) and similar to 60% (red). The S-1 pathway, however, is efficient only for the red carotenoid pool for which two S-1 routes characterized by 0.33 and 2.4 ps time constants were identified. For the blue carotenoids the S-1-mediated pathway is represented only by a minor route likely involving a hot S-1 state. The relaxed S-1 state of blue carotenoids decays to the ground state within 21 ps. Presence of a fraction of non-transferring red carotenoids with the S-1 lifetime of 13 ps indicates some specific carotenoid-protein interaction that must shorten the intrinsic S-1 lifetime of Vio and/or Vau whose S-1 lifetimes in methanol are 26 and 29 ps, respectively. The VCP complex from N. oceanica is the first example of a light-harvesting complex binding only non-carbonyl carotenoids with carotenoid-to-chlorophyll energy transfer efficiency over 90%.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica et Biophysica Acta - Bioenergetics

ISSN

0005-2728

e-ISSN

—

Svazek periodika

1857

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

370-379

Kód UT WoS článku

000372675600006

EID výsledku v databázi Scopus

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