Tracing nitrogen flow in a root-hemiparasitic association by foliar stable-isotope labelling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F16%3A43890631" target="_blank" >RIV/60076658:12310/16:43890631 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ingentaconnect.com/content/botbel/plecevo/2016/00000149/00000001/art00005" target="_blank" >http://www.ingentaconnect.com/content/botbel/plecevo/2016/00000149/00000001/art00005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5091/plecevo.2016.1112" target="_blank" >10.5091/plecevo.2016.1112</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tracing nitrogen flow in a root-hemiparasitic association by foliar stable-isotope labelling

Popis výsledku v původním jazyce

Background and aims - The resource flows in the host-hemiparasite association have been frequently studied by applying stable isotope techniques. However, these methods of artificial labelling required sophisticated equipment preventing their application to field experiments. Here, we aimed to test the applicability of the (NC)-N-15-C-13-urea foliar brushing method in tracing the resource flows between a root hemiparasite, Rhinanthus major, and a host, Triticum aestivum. In addition, the dynamics of the label movement was examined in order to provide an estimate of the most appropriate harvesting time. Methods - Double-labelled urea (98 atom % N-15, 99 atom % C-13) solution (2 g dm(-3)) was applied on host plants grown with hemiparasites by a single foliar brushing. Above- and belowground biomass of both species was harvested 3, 7, and 14 d after host labelling and its isotopic composition was analyzed. Final isotopic enrichment of biomass was expressed as the atom percent difference between labelled samples and the mean of corresponding controls. Key results - Our results showed that a single leaf-brushing with (NC)-N-15-C-13-urea provided sufficiently N-15-labelled plant material, but it was insufficient to shift the natural abundance of C-13 in both species. Similar N-15 values were found for the host and hemiparasite biomass already 3 d after labelling, but the N-15 enrichment of attached hemiparasite significantly increased in time. Within a week, N-15-label gradually dispersed into the host tissues and was simultaneously transferred into the hemiparasite via the root connections. Conclusions - We present foliar brushing by N-15-urea as a simple and precise labelling method, which can be widely applied in both greenhouse and field experiments to examine the nitrogen flows between root hemiparasites and their host species.

Název v anglickém jazyce

Tracing nitrogen flow in a root-hemiparasitic association by foliar stable-isotope labelling

Popis výsledku anglicky

Background and aims - The resource flows in the host-hemiparasite association have been frequently studied by applying stable isotope techniques. However, these methods of artificial labelling required sophisticated equipment preventing their application to field experiments. Here, we aimed to test the applicability of the (NC)-N-15-C-13-urea foliar brushing method in tracing the resource flows between a root hemiparasite, Rhinanthus major, and a host, Triticum aestivum. In addition, the dynamics of the label movement was examined in order to provide an estimate of the most appropriate harvesting time. Methods - Double-labelled urea (98 atom % N-15, 99 atom % C-13) solution (2 g dm(-3)) was applied on host plants grown with hemiparasites by a single foliar brushing. Above- and belowground biomass of both species was harvested 3, 7, and 14 d after host labelling and its isotopic composition was analyzed. Final isotopic enrichment of biomass was expressed as the atom percent difference between labelled samples and the mean of corresponding controls. Key results - Our results showed that a single leaf-brushing with (NC)-N-15-C-13-urea provided sufficiently N-15-labelled plant material, but it was insufficient to shift the natural abundance of C-13 in both species. Similar N-15 values were found for the host and hemiparasite biomass already 3 d after labelling, but the N-15 enrichment of attached hemiparasite significantly increased in time. Within a week, N-15-label gradually dispersed into the host tissues and was simultaneously transferred into the hemiparasite via the root connections. Conclusions - We present foliar brushing by N-15-urea as a simple and precise labelling method, which can be widely applied in both greenhouse and field experiments to examine the nitrogen flows between root hemiparasites and their host species.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/GP14-26779P" target="_blank" >GP14-26779P: Hledání Achillovy paty Calamagrostis epigejos. Můžerme potlačit expanzní trávu pomocí poloparazitických kokrhelů (Rhinanthus)?</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Ecology and Evolution

ISSN

2032-3913

e-ISSN

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Svazek periodika

149

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

6

Strana od-do

39-44

Kód UT WoS článku

000371196200005

EID výsledku v databázi Scopus

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